Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control

Christina Glytsou; Enrique Calvo; Sara Cogliati; Arpit Mehrotra; Irene Anastasia; Giovanni Rigoni; Andrea Raimondi; Norihito Shintani; Marta Loureiro; Jesùs Vazquez; Luca Pellegrini; Jose Antonio Enriquez; Luca Scorrano; Maria Eugenia Soriano

doi:10.1016/j.celrep.2016.11.049

Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control

Christina Glytsou
, Enrique Calvo
, Sara Cogliati
, Arpit Mehrotra
, Irene Anastasia
, Giovanni Rigoni
, Andrea Raimondi
, Norihito Shintani
, Marta Loureiro
, Jesùs Vazquez
, Luca Pellegrini
, Jose Antonio Enriquez
, Luca Scorrano
, Maria Eugenia Soriano

Research output: Contribution to journal › Article › peer-review

Abstract

The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS.

Original language	American English
Pages (from-to)	3024-3034
Number of pages	11
Journal	Cell Reports
Volume	17
Issue number	11
DOIs	https://doi.org/10.1016/j.celrep.2016.11.049
State	Published - Dec 13 2016
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Keywords

MICOS
OPA1
cristae
mitochondria
proteomics

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10.1016/j.celrep.2016.11.049

Cite this

Glytsou, C., Calvo, E., Cogliati, S., Mehrotra, A., Anastasia, I., Rigoni, G., Raimondi, A., Shintani, N., Loureiro, M., Vazquez, J., Pellegrini, L., Enriquez, J. A., Scorrano, L., & Soriano, M. E. (2016). Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control. Cell Reports, 17(11), 3024-3034. https://doi.org/10.1016/j.celrep.2016.11.049

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title = "Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control",

abstract = "The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS.",

keywords = "MICOS, OPA1, cristae, mitochondria, proteomics",

author = "Christina Glytsou and Enrique Calvo and Sara Cogliati and Arpit Mehrotra and Irene Anastasia and Giovanni Rigoni and Andrea Raimondi and Norihito Shintani and Marta Loureiro and Jes{\`u}s Vazquez and Luca Pellegrini and Enriquez, \{Jose Antonio\} and Luca Scorrano and Soriano, \{Maria Eugenia\}",

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month = dec,

day = "13",

doi = "10.1016/j.celrep.2016.11.049",

language = "American English",

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Glytsou, C, Calvo, E, Cogliati, S, Mehrotra, A, Anastasia, I, Rigoni, G, Raimondi, A, Shintani, N, Loureiro, M, Vazquez, J, Pellegrini, L, Enriquez, JA, Scorrano, L & Soriano, ME 2016, 'Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control', Cell Reports, vol. 17, no. 11, pp. 3024-3034. https://doi.org/10.1016/j.celrep.2016.11.049

TY - JOUR

T1 - Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control

AU - Glytsou, Christina

AU - Calvo, Enrique

AU - Cogliati, Sara

AU - Mehrotra, Arpit

AU - Anastasia, Irene

AU - Rigoni, Giovanni

AU - Raimondi, Andrea

AU - Shintani, Norihito

AU - Loureiro, Marta

AU - Vazquez, Jesùs

AU - Pellegrini, Luca

AU - Enriquez, Jose Antonio

AU - Scorrano, Luca

AU - Soriano, Maria Eugenia

PY - 2016/12/13

Y1 - 2016/12/13

N2 - The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS.

AB - The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS.

KW - MICOS

KW - OPA1

KW - cristae

KW - mitochondria

KW - proteomics

UR - https://www.scopus.com/pages/publications/85003955418

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DO - 10.1016/j.celrep.2016.11.049

M3 - Article

C2 - 27974214

SN - 2211-1247

VL - 17

SP - 3024

EP - 3034

JO - Cell Reports

JF - Cell Reports

IS - 11

ER -

Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control

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Keywords

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