Ovotransferrin nanofibril formation in the presence of glycerol or sorbitol

TY - JOUR

T1 - Ovotransferrin nanofibril formation in the presence of glycerol or sorbitol

AU - Wei, Z.

AU - Huang, Qingrong

N1 - Funding Information: This work was supported by United States Department of Agriculture , National Institute of Food and Agriculture (grant No. 2019-67017-29176 ). Financial support from the China Scholarship Council for the first author is acknowledged. Publisher Copyright: © 2019 Elsevier Ltd

PY - 2020/2/1

Y1 - 2020/2/1

N2 - Impact of glycerol and sorbitol on assembly of iron-bound ovotransferrin (OVT) into nanofibrils was investigated. Thioflavin T fluorescence result indicated that the presence of glycerol or sorbitol could reduce the rate of OVT fibrillation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated that 60% sorbitol could retard hydrolysis of OVT completely for a period of time during thermal treatment, and decoupling hydrolysis from fibril self-assembly helped to identify the building blocks of OVT nanofibrils. OVT nanofibrils were composed of both intact OVT monomers and OVT-derived peptides. Influence of glycerol and sorbitol on morphology of OVT nanofibrils was studied using atomic force microscopy. The presence of glycerol or sorbitol shortened OVT nanofibrils, and the presence of 60% glycerol or sorbitol could increase thickness of OVT nanofibrils. Hopefully, this work may provide new insight about building blocks of protein nanofibrils and impact of polyols on protein fibrillation.

AB - Impact of glycerol and sorbitol on assembly of iron-bound ovotransferrin (OVT) into nanofibrils was investigated. Thioflavin T fluorescence result indicated that the presence of glycerol or sorbitol could reduce the rate of OVT fibrillation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated that 60% sorbitol could retard hydrolysis of OVT completely for a period of time during thermal treatment, and decoupling hydrolysis from fibril self-assembly helped to identify the building blocks of OVT nanofibrils. OVT nanofibrils were composed of both intact OVT monomers and OVT-derived peptides. Influence of glycerol and sorbitol on morphology of OVT nanofibrils was studied using atomic force microscopy. The presence of glycerol or sorbitol shortened OVT nanofibrils, and the presence of 60% glycerol or sorbitol could increase thickness of OVT nanofibrils. Hopefully, this work may provide new insight about building blocks of protein nanofibrils and impact of polyols on protein fibrillation.

KW - Atomic force microscopy

KW - Building blocks

KW - Glycerol

KW - Hydrolysis

KW - Ovotransferrin nanofibril

KW - Sorbitol

UR - http://www.scopus.com/inward/record.url?scp=85071856559&partnerID=8YFLogxK

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U2 - https://doi.org/10.1016/j.foodchem.2019.125453

DO - https://doi.org/10.1016/j.foodchem.2019.125453

M3 - Article

C2 - 31505417

SN - 0308-8146

VL - 305

JO - Food Chemistry

JF - Food Chemistry

M1 - 125453

ER -