Abstract
The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 98-105 |
| Number of pages | 8 |
| Journal | Bioresource Technology |
| Volume | 240 |
| DOIs | |
| State | Published - Jan 1 2017 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Bioengineering
- Waste Management and Disposal
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
Keywords
- Dehalococcoides mccartyi
- Non-dechlorinators
- Oxygen
- Trichloroethylene
Cite this
}
Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium. / Liu, Na; Li, Haijun; Li, Mengyan; Ding, Longzhen; Weng, Chih Huang; Dong, Cheng Di.
In: Bioresource Technology, Vol. 240, 01.01.2017, p. 98-105.Research output: Contribution to journal › Article
TY - JOUR
T1 - Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium
AU - Liu, Na
AU - Li, Haijun
AU - Li, Mengyan
AU - Ding, Longzhen
AU - Weng, Chih Huang
AU - Dong, Cheng Di
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.
AB - The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.
KW - Dehalococcoides mccartyi
KW - Non-dechlorinators
KW - Oxygen
KW - Trichloroethylene
UR - http://www.scopus.com/inward/record.url?scp=85014494218&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014494218&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.biortech.2017.02.112
DO - https://doi.org/10.1016/j.biortech.2017.02.112
M3 - Article
C2 - 28274623
VL - 240
SP - 98
EP - 105
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -