Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium

Na Liu, Haijun Li, Mengyan Li, Longzhen Ding, Chih Huang Weng, Cheng Di Dong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.

Original languageEnglish (US)
Pages (from-to)98-105
Number of pages8
JournalBioresource Technology
Volume240
DOIs
StatePublished - Jan 1 2017

Fingerprint

Trichloroethylene
Dechlorination
dechlorination
trichloroethylene
Oxygen
oxygen
Genes
biotransformation
gene
ethylene
Bacteria
Throughput
exposure
effect
bacterium

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Waste Management and Disposal
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment

Keywords

  • Dehalococcoides mccartyi
  • Non-dechlorinators
  • Oxygen
  • Trichloroethylene

Cite this

Liu, Na ; Li, Haijun ; Li, Mengyan ; Ding, Longzhen ; Weng, Chih Huang ; Dong, Cheng Di. / Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium. In: Bioresource Technology. 2017 ; Vol. 240. pp. 98-105.
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Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium. / Liu, Na; Li, Haijun; Li, Mengyan; Ding, Longzhen; Weng, Chih Huang; Dong, Cheng Di.

In: Bioresource Technology, Vol. 240, 01.01.2017, p. 98-105.

Research output: Contribution to journalArticle

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