PCR detection of Helicobacter pylori in string-absorbed gastric juice

Maria G. Domínguez-Bello; Calixto Cienfuentes; Roberto Romero; Paul García; Ivette Gómez; Vladimir Mago; Nelson Reyes; P. Gueneau De Novoa

doi:https://doi.org/10.1016/S0378-1097(01)00120-3

PCR detection of Helicobacter pylori in string-absorbed gastric juice

Maria G. Domínguez-Bello, Calixto Cienfuentes, Roberto Romero, Paul García, Ivette Gómez, Vladimir Mago, Nelson Reyes, P. Gueneau De Novoa

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.

Original language	English (US)
Pages (from-to)	15-16
Number of pages	2
Journal	FEMS Microbiology Letters
Volume	198
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(01)00120-3
State	Published - Apr 20 2001
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Keywords

Diagnostic
Gastric juice
Helicobacter pylori
PCR

Access to Document

https://doi.org/10.1016/S0378-1097(01)00120-3

Cite this

@article{2707cf33ce3c41559d6fa52f729d31e4,

title = "PCR detection of Helicobacter pylori in string-absorbed gastric juice",

abstract = "Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.",

keywords = "Diagnostic, Gastric juice, Helicobacter pylori, PCR",

author = "Dom{\'i}nguez-Bello, {Maria G.} and Calixto Cienfuentes and Roberto Romero and Paul Garc{\'i}a and Ivette G{\'o}mez and Vladimir Mago and Nelson Reyes and {Gueneau De Novoa}, P.",

year = "2001",

month = apr,

day = "20",

doi = "https://doi.org/10.1016/S0378-1097(01)00120-3",

language = "English (US)",

volume = "198",

pages = "15--16",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - PCR detection of Helicobacter pylori in string-absorbed gastric juice

AU - Domínguez-Bello, Maria G.

AU - Cienfuentes, Calixto

AU - Romero, Roberto

AU - García, Paul

AU - Gómez, Ivette

AU - Mago, Vladimir

AU - Reyes, Nelson

AU - Gueneau De Novoa, P.

PY - 2001/4/20

Y1 - 2001/4/20

N2 - Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.

AB - Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.

KW - Diagnostic

KW - Gastric juice

KW - Helicobacter pylori

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=0035917507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035917507&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/S0378-1097(01)00120-3

DO - https://doi.org/10.1016/S0378-1097(01)00120-3

M3 - Article

C2 - 11325547

VL - 198

SP - 15

EP - 16

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -

PCR detection of Helicobacter pylori in string-absorbed gastric juice

Abstract

ASJC Scopus subject areas

Keywords

Access to Document

Other files and links

Fingerprint

Cite this