PCR detection of Helicobacter pylori in string-absorbed gastric juice

Maria G. Domínguez-Bello, Calixto Cienfuentes, Roberto Romero, Paul García, Ivette Gómez, Vladimir Mago, Nelson Reyes, P. Gueneau De Novoa

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.

Original languageEnglish (US)
Pages (from-to)15-16
Number of pages2
JournalFEMS Microbiology Letters
Volume198
Issue number1
DOIs
StatePublished - Apr 20 2001
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Keywords

  • Diagnostic
  • Gastric juice
  • Helicobacter pylori
  • PCR

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