Pelvic organ prolapse is associated with alteration of sphingosine-1-phosphate/Rho-kinase signalling pathway in human vaginal wall

S. H. Rhee; P. Zhang; K. Hunter; S. T. Mama; R. Caraballo; A. S. Holzberg; R. H. Seftel; A. D. Seftel; K. T. Echols; M. E. DiSanto

doi:10.3109/01443615.2015.1004527

Pelvic organ prolapse is associated with alteration of sphingosine-1-phosphate/Rho-kinase signalling pathway in human vaginal wall

S. H. Rhee
, P. Zhang
, K. Hunter
, S. T. Mama
, R. Caraballo
, A. S. Holzberg
, R. H. Seftel
, A. D. Seftel
, K. T. Echols
, M. E. DiSanto

CMSRU Biomedical Science

Rowan University

Research output: Contribution to journal › Article › peer-review

Abstract

Pelvic organ prolapse (POP) is a debilitating condition of unknown aetiology affecting > 50% of women over 40 years of age. In POP patients, the vaginal walls are weakened allowing descent of pelvic organs through the vagina. We sought to determine if sphingosine-1-phosphate (S1P) signalling, which regulates smooth muscle contractility and apoptosis via the RhoA/Rho-kinase (ROK) pathway, is altered in the vagina of women with POP. Utilising anterior vaginal wall specimens, we provide novel demonstration of the S1P pathway in this organ. Additionally, comparing specimens from women having pelvic reconstructive surgery for POP and control subjects, we reveal increases in mRNA expression of the three major mammalian S1P receptors (S1P1-S1P3), and RhoA and the ROK isoforms: ROKα and ROKβ in POP patients, which correlates with a decrease in elastic fibre assembly pathway constituents. Taken together, our data suggest the S1P/ROK pathway as a novel area for future POP research and potential therapeutic development.

Original language	American English
Pages (from-to)	726-732
Number of pages	7
Journal	Journal of Obstetrics and Gynaecology
Volume	35
Issue number	7
DOIs	https://doi.org/10.3109/01443615.2015.1004527
State	Published - Oct 3 2015

ASJC Scopus subject areas

Obstetrics and Gynecology

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Rhee, S. H., Zhang, P., Hunter, K., Mama, S. T., Caraballo, R., Holzberg, A. S., Seftel, R. H., Seftel, A. D., Echols, K. T., & DiSanto, M. E. (2015). Pelvic organ prolapse is associated with alteration of sphingosine-1-phosphate/Rho-kinase signalling pathway in human vaginal wall. Journal of Obstetrics and Gynaecology, 35(7), 726-732. https://doi.org/10.3109/01443615.2015.1004527

@article{fe08ad53fe16412bad21b879b3814cfa,

title = "Pelvic organ prolapse is associated with alteration of sphingosine-1-phosphate/Rho-kinase signalling pathway in human vaginal wall",

abstract = "Pelvic organ prolapse (POP) is a debilitating condition of unknown aetiology affecting > 50\% of women over 40 years of age. In POP patients, the vaginal walls are weakened allowing descent of pelvic organs through the vagina. We sought to determine if sphingosine-1-phosphate (S1P) signalling, which regulates smooth muscle contractility and apoptosis via the RhoA/Rho-kinase (ROK) pathway, is altered in the vagina of women with POP. Utilising anterior vaginal wall specimens, we provide novel demonstration of the S1P pathway in this organ. Additionally, comparing specimens from women having pelvic reconstructive surgery for POP and control subjects, we reveal increases in mRNA expression of the three major mammalian S1P receptors (S1P1-S1P3), and RhoA and the ROK isoforms: ROKα and ROKβ in POP patients, which correlates with a decrease in elastic fibre assembly pathway constituents. Taken together, our data suggest the S1P/ROK pathway as a novel area for future POP research and potential therapeutic development.",

author = "Rhee, \{S. H.\} and P. Zhang and K. Hunter and Mama, \{S. T.\} and R. Caraballo and Holzberg, \{A. S.\} and Seftel, \{R. H.\} and Seftel, \{A. D.\} and Echols, \{K. T.\} and DiSanto, \{M. E.\}",

note = "Publisher Copyright: {\textcopyright} 2015 {\textcopyright} 2015 Taylor \& Francis Group, LLC.",

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month = oct,

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doi = "10.3109/01443615.2015.1004527",

language = "American English",

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Rhee, SH, Zhang, P, Hunter, K, Mama, ST, Caraballo, R, Holzberg, AS, Seftel, RH, Seftel, AD, Echols, KT & DiSanto, ME 2015, 'Pelvic organ prolapse is associated with alteration of sphingosine-1-phosphate/Rho-kinase signalling pathway in human vaginal wall', Journal of Obstetrics and Gynaecology, vol. 35, no. 7, pp. 726-732. https://doi.org/10.3109/01443615.2015.1004527

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AU - Rhee, S. H.

AU - Zhang, P.

AU - Hunter, K.

AU - Mama, S. T.

AU - Caraballo, R.

AU - Holzberg, A. S.

AU - Seftel, R. H.

AU - Seftel, A. D.

AU - Echols, K. T.

AU - DiSanto, M. E.

PY - 2015/10/3

Y1 - 2015/10/3

N2 - Pelvic organ prolapse (POP) is a debilitating condition of unknown aetiology affecting > 50% of women over 40 years of age. In POP patients, the vaginal walls are weakened allowing descent of pelvic organs through the vagina. We sought to determine if sphingosine-1-phosphate (S1P) signalling, which regulates smooth muscle contractility and apoptosis via the RhoA/Rho-kinase (ROK) pathway, is altered in the vagina of women with POP. Utilising anterior vaginal wall specimens, we provide novel demonstration of the S1P pathway in this organ. Additionally, comparing specimens from women having pelvic reconstructive surgery for POP and control subjects, we reveal increases in mRNA expression of the three major mammalian S1P receptors (S1P1-S1P3), and RhoA and the ROK isoforms: ROKα and ROKβ in POP patients, which correlates with a decrease in elastic fibre assembly pathway constituents. Taken together, our data suggest the S1P/ROK pathway as a novel area for future POP research and potential therapeutic development.

AB - Pelvic organ prolapse (POP) is a debilitating condition of unknown aetiology affecting > 50% of women over 40 years of age. In POP patients, the vaginal walls are weakened allowing descent of pelvic organs through the vagina. We sought to determine if sphingosine-1-phosphate (S1P) signalling, which regulates smooth muscle contractility and apoptosis via the RhoA/Rho-kinase (ROK) pathway, is altered in the vagina of women with POP. Utilising anterior vaginal wall specimens, we provide novel demonstration of the S1P pathway in this organ. Additionally, comparing specimens from women having pelvic reconstructive surgery for POP and control subjects, we reveal increases in mRNA expression of the three major mammalian S1P receptors (S1P1-S1P3), and RhoA and the ROK isoforms: ROKα and ROKβ in POP patients, which correlates with a decrease in elastic fibre assembly pathway constituents. Taken together, our data suggest the S1P/ROK pathway as a novel area for future POP research and potential therapeutic development.

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JO - Journal of Obstetrics and Gynaecology

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ER -

Pelvic organ prolapse is associated with alteration of sphingosine-1-phosphate/Rho-kinase signalling pathway in human vaginal wall

Abstract

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