Phenotype of columnar-lined esophagus in rats with esophagogastroduodenal anastomosis

Similarity to human Barrett's esophagus

Yinghao Su, Xiaoxin Chen, Michael Klein, Mingzhu Fang, Su Wang, Chung Yang, Raj K. Goyal

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

In rats, esophagogastroduodenal anastomosis (EGDA) without concomitant chemical carcinogen treatment can lead to columnar-lined esophagus (CLE) including metaplasia, dysplasia, and esophageal adenocarcinoma (EAC). This study describes the morphology and phenotypic features of CLE and EAC in the rat model and compares them with the corresponding lesions in human Barrett's esophagus (BE). Swiss roll preparations of esophagi of EGDA rats and biopsies from human BE containing specialized intestinal metaplasia (SIM) and EAC were examined. The esophagi of EGDA rats showed esophagitis, CLE, islands of multilayered epithelium (MLE), dysplasia and EAC. The CLE had features of specialized intestinal metaplasia. MLE frequently occurred at the neo-squamocolumnar junction and occasionally in the mid-esophagus in isolated foci. Scattered mucinous cells in esophageal squamous epithelium were also found. The CLE and MLE in EGDA rats resembled the lesions described in human BE in morphology, mucin features and expression of differentiation markers (CK7, CK20, Das-1, villin, and pS2/TFF1). Invasive EAC in EGDA rat is of well-differentiated mucinous type, which is in contrast to the variably differentiated glandular type of adenocarcinoma in human BE. p53, c-myc, and cyclooxygenase 2 are expressed in both the rat and human SIM and EAC. These studies indicate that, not withstanding small differences, SIM and EAC induced in EGDA rats are similar to the corresponding lesions in human BE. EGDA rats may serve as a useful model to study the pathogenesis, molecular biology, and chemopreventive interventions of human BE and EAC.

Original languageEnglish (US)
Pages (from-to)753-765
Number of pages13
JournalLaboratory Investigation
Volume84
Issue number6
DOIs
StatePublished - Jun 1 2004

Fingerprint

Barrett Esophagus
Esophagus
Adenocarcinoma
Phenotype
Metaplasia
Epithelium
Esophagitis
Differentiation Antigens
Mucins
Cyclooxygenase 2
Islands
Carcinogens
Molecular Biology
Biopsy

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pathology and Forensic Medicine
  • Cell Biology

Keywords

  • Esophageal adenocarcinoma
  • Intestinal metaplasia
  • Phenotype of columnar-lined esophagus

Cite this

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title = "Phenotype of columnar-lined esophagus in rats with esophagogastroduodenal anastomosis: Similarity to human Barrett's esophagus",
abstract = "In rats, esophagogastroduodenal anastomosis (EGDA) without concomitant chemical carcinogen treatment can lead to columnar-lined esophagus (CLE) including metaplasia, dysplasia, and esophageal adenocarcinoma (EAC). This study describes the morphology and phenotypic features of CLE and EAC in the rat model and compares them with the corresponding lesions in human Barrett's esophagus (BE). Swiss roll preparations of esophagi of EGDA rats and biopsies from human BE containing specialized intestinal metaplasia (SIM) and EAC were examined. The esophagi of EGDA rats showed esophagitis, CLE, islands of multilayered epithelium (MLE), dysplasia and EAC. The CLE had features of specialized intestinal metaplasia. MLE frequently occurred at the neo-squamocolumnar junction and occasionally in the mid-esophagus in isolated foci. Scattered mucinous cells in esophageal squamous epithelium were also found. The CLE and MLE in EGDA rats resembled the lesions described in human BE in morphology, mucin features and expression of differentiation markers (CK7, CK20, Das-1, villin, and pS2/TFF1). Invasive EAC in EGDA rat is of well-differentiated mucinous type, which is in contrast to the variably differentiated glandular type of adenocarcinoma in human BE. p53, c-myc, and cyclooxygenase 2 are expressed in both the rat and human SIM and EAC. These studies indicate that, not withstanding small differences, SIM and EAC induced in EGDA rats are similar to the corresponding lesions in human BE. EGDA rats may serve as a useful model to study the pathogenesis, molecular biology, and chemopreventive interventions of human BE and EAC.",
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Phenotype of columnar-lined esophagus in rats with esophagogastroduodenal anastomosis : Similarity to human Barrett's esophagus. / Su, Yinghao; Chen, Xiaoxin; Klein, Michael; Fang, Mingzhu; Wang, Su; Yang, Chung; Goyal, Raj K.

In: Laboratory Investigation, Vol. 84, No. 6, 01.06.2004, p. 753-765.

Research output: Contribution to journalArticle

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T1 - Phenotype of columnar-lined esophagus in rats with esophagogastroduodenal anastomosis

T2 - Similarity to human Barrett's esophagus

AU - Su, Yinghao

AU - Chen, Xiaoxin

AU - Klein, Michael

AU - Fang, Mingzhu

AU - Wang, Su

AU - Yang, Chung

AU - Goyal, Raj K.

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