Polyadenylation and degradation of incomplete RNA polymerase i transcripts in mammalian cells

Natalia Shcherbik, Minshi Wang, Yevgeniya R. Lapik, Leena Srivastava, Dimitri Pestov

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Most transcripts in growing cells are ribosomal RNA precursors (pre-rRNA). Here, we show that in mammals, aberrant pre-rRNA transcripts generated by RNA polymerase I (Pol I) are polyadenylated and accumulate markedly after treatment with low concentrations of actinomycin D (ActD), which blocks the synthesis of full-length rRNA. The poly(A) polymerase-associated domain-containing protein 5 is required for polyadenylation, whereas the exosome is partly responsible for the degradation of the short aberrant transcripts. Thus, polyadenylation functions in the quality control of Pol I transcription in metazoan cells. The impact of excessive aberrant RNAs on the degradation machinery is an unrecognized mechanism that might contribute to biological properties of ActD.

Original languageEnglish (US)
Pages (from-to)106-111
Number of pages6
JournalEMBO Reports
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2010

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Polyadenylation
RNA Precursors
DNA-Directed RNA Polymerases
Cells
Dactinomycin
Degradation
Polynucleotide Adenylyltransferase
Exosomes
RNA Polymerase I
Mammals
RNA Stability
Transcription
Quality Control
Machinery
Quality control
RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology
  • Biochemistry

Cite this

Shcherbik, Natalia ; Wang, Minshi ; Lapik, Yevgeniya R. ; Srivastava, Leena ; Pestov, Dimitri. / Polyadenylation and degradation of incomplete RNA polymerase i transcripts in mammalian cells. In: EMBO Reports. 2010 ; Vol. 11, No. 2. pp. 106-111.
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Polyadenylation and degradation of incomplete RNA polymerase i transcripts in mammalian cells. / Shcherbik, Natalia; Wang, Minshi; Lapik, Yevgeniya R.; Srivastava, Leena; Pestov, Dimitri.

In: EMBO Reports, Vol. 11, No. 2, 01.02.2010, p. 106-111.

Research output: Contribution to journalArticle

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