Polyethylene glycol protects primary hepatocytes during supercooling preservation

C. F. Puts, T. A. Berendsen, B. G. Bruinsma, Sinan Ozer, Martha Luitje, O. Berk Usta, M. L. Yarmush, K. Uygun

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Cold storage (at 4. °C) offers a compromise between the benefits and disadvantages of cooling. It allows storage of organs or cells for later use that would otherwise quickly succumb to warm ischemia, but comprises cold ischemia that, when not controlled properly, can result in severe damage as well by both similar and unique mechanisms. We hypothesized that polyethylene glycol (PEG) 35. kDa would ameliorate these injury pathways and improve cold primary hepatocyte preservation. We show that reduction of the storage temperature to below zero by means of supercooling, or subzero non-freezing, together with PEG supplementation increases the viable storage time of primary rat hepatocytes in University of Wisconsin (UW) solution from 1. day to 4. days. We find that the addition of 5% PEG 35. kDa to the storage medium prevents cold-induced lipid peroxidation and maintains hepatocyte viability and functionality during storage. These results suggest that PEG supplementation in combination with supercooling may enable a more optimized cell and organ preservation.

Original languageEnglish (US)
Pages (from-to)125-129
Number of pages5
JournalCryobiology
Volume71
Issue number1
DOIs
StatePublished - Aug 1 2015

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Hepatocytes
  • Lipid peroxidation
  • Polyethylene glycol
  • Preservation
  • Supercooling
  • University of Wisconsin solution

Fingerprint

Dive into the research topics of 'Polyethylene glycol protects primary hepatocytes during supercooling preservation'. Together they form a unique fingerprint.

Cite this