TY - JOUR
T1 - Prenylated Coumaric Acids fromArtemisia scopariaBeneficially Modulate Adipogenesis
AU - Ribnicky, David
AU - Beom Kim, Seon
AU - Poulev, Alexander
AU - Wang, Yang
AU - Boudreau, Anik
AU - Raskin, Ilya
AU - Bisson, Jonathan
AU - Ray, G. Joseph
AU - Chen, Shao Nong
AU - Richard, Allison
AU - Stephens, Jacqueline M.
AU - Pauli, Guido F.
N1 - Funding Information: This work was supported by the Office of Dietary Supplements and the National Center for Complementary and Integrative Health, both of the National Institutes of Health (NIH), under the two awards: P50 AT002776, funding the Botanical Dietary Supplements Research Center of Pennington Biomedical Research Center and the Department of Plant Biology and Pathology in the School of Environmental and Biological Sciences (SEBS) of Rutgers University and U41 AT008706, supporting the CENAPT at the University of Illinois at Chicago. The authors are also grateful for support by the New Jersey Agricultural Experiment Station (NJAES) at Rutgers University. Additional support was provided by the Fogarty International Center of NIH award U01 TW006674. Publisher Copyright: © 2021 American Chemical Society and American Society of Pharmacognosy
PY - 2021/4/23
Y1 - 2021/4/23
N2 - Two new diprenylated coumaric acid isomers (1aand1b) and two known congeners, capillartemisin A (2) and B (3), were isolated fromArtemisia scopariaas bioactive markers using bioactivity-guided HPLC fractionation. Their structures were determined by spectroscopic means, including 1D and 2D NMR methods and LC-MS, with their purity assessed by 1D1H pure shift qNMR spectroscopic analysis. The bioactivity of compounds was evaluated by enhanced accumulation of lipids, as measured using Oil Red O staining, and by increased expression of several adipocyte marker genes, including adiponectin in 3T3-L1 adipocytes relative to untreated negative controls. Compared to the plant’s 80% EtOH extract, these purified compounds showed significant but still weaker inhibition of TNFα-induced lipolysis in 3T3-L1 adipocytes. This suggests that additional bioactive substances are responsible for the multiple metabolically favorable effects on adipocytes observed withArtemisia scopariaextract.
AB - Two new diprenylated coumaric acid isomers (1aand1b) and two known congeners, capillartemisin A (2) and B (3), were isolated fromArtemisia scopariaas bioactive markers using bioactivity-guided HPLC fractionation. Their structures were determined by spectroscopic means, including 1D and 2D NMR methods and LC-MS, with their purity assessed by 1D1H pure shift qNMR spectroscopic analysis. The bioactivity of compounds was evaluated by enhanced accumulation of lipids, as measured using Oil Red O staining, and by increased expression of several adipocyte marker genes, including adiponectin in 3T3-L1 adipocytes relative to untreated negative controls. Compared to the plant’s 80% EtOH extract, these purified compounds showed significant but still weaker inhibition of TNFα-induced lipolysis in 3T3-L1 adipocytes. This suggests that additional bioactive substances are responsible for the multiple metabolically favorable effects on adipocytes observed withArtemisia scopariaextract.
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U2 - https://doi.org/10.1021/acs.jnatprod.0c01149
DO - https://doi.org/10.1021/acs.jnatprod.0c01149
M3 - Review article
C2 - 33830759
SN - 0163-3864
VL - 84
SP - 1078
EP - 1086
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 4
ER -