Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid

Zoltán Spolarics, István Mucha, József Mandl, Raymund Machovich, Gábor Bánhegyi, Ferenc Antonio, Tamáas Garzó

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A2 (TXB2) of prostacyclin (6-keto PGF1∝) and one of the prostaglandins (PGF2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB2, while smaller amounts of 6-keto PGF1∝ and PGF2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB2 production and provoked the synthesis of PGF2∝ and 6-keto PGF1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10-5 M. Higher concentrations further increased TXB2 production and also increased the synthesis of 6-keto PGF1∝ and PGF2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10-7-10-5M arachidonate increased the TXB2, 6-keto PGF1∝ and PGF2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.

Original languageEnglish (US)
Pages (from-to)113-127
Number of pages15
JournalProstaglandins, Leukotrienes and Medicine
Volume29
Issue number2-3
DOIs
StatePublished - Oct 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Biochemistry
  • Physiology

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