Proteomic biomarkers of intra-amniotic inflammation: Relationship with funisitis and early-onset sepsis in the premature neonate

TY - JOUR

T1 - Proteomic biomarkers of intra-amniotic inflammation

T2 - Relationship with funisitis and early-onset sepsis in the premature neonate

AU - Buhimschi, Catalin S.

AU - Buhimschi, Irina A.

AU - Abdel-Razeq, Sonya

AU - Rosenberg, Victor A.

AU - Thung, Stephen F.

AU - Zhao, Guomao

AU - Wang, Erica

AU - Bhandari, Vineet

PY - 2007/3

Y1 - 2007/3

N2 - Our goal was to determine the relationship between 4 amniotic fluid (AF) proteomic biomarkers (human neutrophil defensins 2 and 1, calgranulins C and A) characteristic of intra-amniotic inflammation, and funisitis and early-onset sepsis in premature neonates. The mass restricted (MR) score was generated from AF obtained from women in preterm labor (n = 123). The MR score ranged from 0-4 (none to all biomarkers present). Funisitis was graded histologically and interpreted in relation to the MR scores. Neonates (n = 97) were evaluated for early-onset sepsis. There was significant correlation between the severity of AF inflammation and the presence (53/123) and grades of funisitis (p < 0.001). Funisitis occurred independently of the amniocentesis-to-delivery interval or status of the membranes and was best predicted by an MR score 3-4 and an earlier gestational age (GA) at delivery. Neonates born to women with an MR score 3-4 had an increased incidence of suspected/confirmed sepsis, even after adjusting for GA at birth. Calgranulin C had the highest association with clinically significant funisitis, while calgranulin A had the strongest association with early-onset sepsis. To conclude, AF proteomic analysis shows that women with MR scores 3-4 are more likely to have histologic funisitis, and deliver neonates with early-onset sepsis.

AB - Our goal was to determine the relationship between 4 amniotic fluid (AF) proteomic biomarkers (human neutrophil defensins 2 and 1, calgranulins C and A) characteristic of intra-amniotic inflammation, and funisitis and early-onset sepsis in premature neonates. The mass restricted (MR) score was generated from AF obtained from women in preterm labor (n = 123). The MR score ranged from 0-4 (none to all biomarkers present). Funisitis was graded histologically and interpreted in relation to the MR scores. Neonates (n = 97) were evaluated for early-onset sepsis. There was significant correlation between the severity of AF inflammation and the presence (53/123) and grades of funisitis (p < 0.001). Funisitis occurred independently of the amniocentesis-to-delivery interval or status of the membranes and was best predicted by an MR score 3-4 and an earlier gestational age (GA) at delivery. Neonates born to women with an MR score 3-4 had an increased incidence of suspected/confirmed sepsis, even after adjusting for GA at birth. Calgranulin C had the highest association with clinically significant funisitis, while calgranulin A had the strongest association with early-onset sepsis. To conclude, AF proteomic analysis shows that women with MR scores 3-4 are more likely to have histologic funisitis, and deliver neonates with early-onset sepsis.

UR - https://www.scopus.com/pages/publications/33847226584

UR - https://www.scopus.com/pages/publications/33847226584#tab=citedBy

U2 - 10.1203/01.pdr.0000252439.48564.37

DO - 10.1203/01.pdr.0000252439.48564.37

M3 - Article

C2 - 17314690

SN - 0031-3998

VL - 61

SP - 318

EP - 324

JO - Pediatric Research

JF - Pediatric Research

IS - 3

ER -