Protocol for immunofluorescence staining of murine helminth-infected intestinal and lung tissues

Darine W. El-Naccache; Fei Chen; William C. Gause

doi:10.1016/j.xpro.2023.102208

Protocol for immunofluorescence staining of murine helminth-infected intestinal and lung tissues

Darine W. El-Naccache, Fei Chen, William C. Gause

New Jersey Medical School (NJMS)

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

Abstract

Preserving structurally intact tissue through proper tissue fixation and cryopreservation minimizes tissue autolysis, desiccation, and enzymatic degradation. In this protocol, we describe the use of an optimized fresh freezing technique that cryopreserves the tissue and favors the retention of the natural protein structure of antigens. We also detail the use of cold, low-concentration paraformaldehyde (PFA) fixation to enhance tissue morphology and detection of fluorescent proteins, such as GFP and TdTomato, in tissues of genetically engineered mice. For complete details on the use and execution of this protocol, please refer to Chen et al. (2022)¹ and El-Naccache et al. (2022).²

Original language	American English
Article number	102208
Journal	STAR Protocols
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2023.102208
State	Published - Jun 16 2023

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

Keywords

Immunology
Microscopy

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10.1016/j.xpro.2023.102208

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AB - Preserving structurally intact tissue through proper tissue fixation and cryopreservation minimizes tissue autolysis, desiccation, and enzymatic degradation. In this protocol, we describe the use of an optimized fresh freezing technique that cryopreserves the tissue and favors the retention of the natural protein structure of antigens. We also detail the use of cold, low-concentration paraformaldehyde (PFA) fixation to enhance tissue morphology and detection of fluorescent proteins, such as GFP and TdTomato, in tissues of genetically engineered mice. For complete details on the use and execution of this protocol, please refer to Chen et al. (2022)1 and El-Naccache et al. (2022).2

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Protocol for immunofluorescence staining of murine helminth-infected intestinal and lung tissues

Abstract

ASJC Scopus subject areas

Keywords

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