Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120.

G. J. Schneider, Nilgun Tumer, C. Richaud, G. Borbely, R. Haselkorn

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A procedure for the purification of RNA polymerase from vegetative cells of the filamentous cyanobacterium Anabaena 7120 is described. Polyethyleneimine precipitation followed by gel filtration and affinity chromatography steps results in greater than 99% purification with 46% yield. The enzyme has a novel core component of Mr = 66,000, designated gamma, in addition to the typical prokaryotic beta'beta alpha 2 core enzyme. The sigma subunit has been identified by reconstitution of specific transcriptional activity from core enzyme and gel-purified sigma. In transcription assays, this RNA polymerase initiates at a number of Anabaena vegetative cell promoters, as well as from a bacteriophage T4 early promoter, but does not initiate at nitrogen fixation (nif) promoters used in heterocysts. The promoter specificity of Anabaena RNA polymerase is compared with that of Escherichia coli RNA polymerase.

Original languageEnglish (US)
Pages (from-to)14633-14639
Number of pages7
JournalThe Journal of biological chemistry
Volume262
Issue number30
StatePublished - Jan 1 1987
Externally publishedYes

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Anabaena
Cyanobacteria
DNA-Directed RNA Polymerases
Purification
Enzymes
Gels
Nitrogen fixation
Sigma Factor
Affinity chromatography
Polyethyleneimine
Bacteriophage T4
Nitrogen Fixation
Bacteriophages
Transcription
Affinity Chromatography
Escherichia coli
Gel Chromatography
Assays

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Schneider, G. J., Tumer, N., Richaud, C., Borbely, G., & Haselkorn, R. (1987). Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120. The Journal of biological chemistry, 262(30), 14633-14639.
Schneider, G. J. ; Tumer, Nilgun ; Richaud, C. ; Borbely, G. ; Haselkorn, R. / Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120. In: The Journal of biological chemistry. 1987 ; Vol. 262, No. 30. pp. 14633-14639.
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Schneider, GJ, Tumer, N, Richaud, C, Borbely, G & Haselkorn, R 1987, 'Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120.', The Journal of biological chemistry, vol. 262, no. 30, pp. 14633-14639.

Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120. / Schneider, G. J.; Tumer, Nilgun; Richaud, C.; Borbely, G.; Haselkorn, R.

In: The Journal of biological chemistry, Vol. 262, No. 30, 01.01.1987, p. 14633-14639.

Research output: Contribution to journalArticle

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