@inbook{0ca791663f0348ef9ce51d7e57c9351f,
title = "Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy",
abstract = "Plasma membranes are flexible and can exhibit numerous shapes below the optical diffraction limit. The shape of cell periphery can either induce or be a product of local protein density changes, encoding numerous cellular functions. However, quantifying membrane curvature and the ensuing sorting of proteins in live cells remains technically demanding. Here, we demonstrate the use of simple widefield fluorescence microscopy to study the geometrical properties (i.e., radius, length, and number) of thin membrane protrusions. Importantly, the quantification of protrusion radius establishes a platform for studying the curvature preferences of membrane proteins.",
keywords = "Cell membrane, Filopodia, Fluorescence microscopy, Image analysis, Membrane curvature, Protein sorting",
author = "Shilong Yang and Zheng Shi",
note = "Publisher Copyright: {\textcopyright} 2024",
year = "2024",
month = jan,
doi = "10.1016/bs.mie.2024.01.023",
language = "American English",
isbn = "9780443293047",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "385--411",
booktitle = "Biophysical Approaches for the Study of Membrane Structure - Part A",
address = "United States",
}