Quantifying nonspecific tem β-lactamase (blatem) genes in a wastewater stream

Karen L. Lachmayr, Colleen M. Cavanaugh, Lee J. Kerkhof, A. Gregory DiRienzo, Timothy E. Ford

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

To control the antibiotic resistance epidemic, it is necessary to understand the distribution of genetic material encoding antibiotic resistance in the environment and how anthropogenic inputs, such as wastewater, affect this distribution. Approximately two-thirds of antibiotics administered to humans are β-lactams, for which the predominant bacterial resistance mechanism is hydrolysis by β-lactamases. Of the β-lactamases, the TEM family is of overriding significance with regard to diversity, prevalence, and distribution. This paper describes the design of DNA probes universal for all known TEM β-lactamase genes and the application of a quantitative PCR assay (also known as Taqman) to quantify these genes in environmental samples. The primer set was used to study whether sewage, both treated and untreated, contributes to the spread of these genes in receiving waters. It was found that while modern sewage treatment technologies reduce the concentrations of these antibiotic resistance genes, the ratio of blaTEM genes to 16S rRNA genes increases with treatment, suggesting that bacteria harboring blaTEM are more likely to survive the treatment process. Thus, β-lactamase genes are being introduced into the environment in significantly higher concentrations than occur naturally, creating reservoirs of increased resistance potential.

Original languageEnglish (US)
Pages (from-to)203-211
Number of pages9
JournalApplied and environmental microbiology
Volume75
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Waste Water
Genes
Microbial Drug Resistance
Sewage
Lactams
DNA Probes
rRNA Genes
Hydrolysis
Anti-Bacterial Agents
Technology
Bacteria
Polymerase Chain Reaction
Water

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Lachmayr, Karen L. ; Cavanaugh, Colleen M. ; Kerkhof, Lee J. ; DiRienzo, A. Gregory ; Ford, Timothy E. / Quantifying nonspecific tem β-lactamase (blatem) genes in a wastewater stream. In: Applied and environmental microbiology. 2009 ; Vol. 75, No. 1. pp. 203-211.
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Quantifying nonspecific tem β-lactamase (blatem) genes in a wastewater stream. / Lachmayr, Karen L.; Cavanaugh, Colleen M.; Kerkhof, Lee J.; DiRienzo, A. Gregory; Ford, Timothy E.

In: Applied and environmental microbiology, Vol. 75, No. 1, 01.01.2009, p. 203-211.

Research output: Contribution to journalArticle

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