Reverse-phase high-performance liquid chromatography (HPLC) analysis of retinol and retinyl esters in mouse serum and tissues

Youn Kyung Kim, Loredana Quadro

Research output: Chapter in Book/Report/Conference proceedingChapter

37 Scopus citations

Abstract

The ability to measure endogenous metabolites of retinoids (vitamin A and its derivatives) in biological samples is key to understanding the crucial physiological actions of vitamin A. Over the years, many assays and methods have been developed to analyze different retinoids in biological samples. Liquid chromatography is the best analytical technique for routine analysis of these compounds. However, due to their different chemical properties, different retinoid metabolites cannot be accurately separated and quantified in a single chromatographic run. Here, we will describe a reverse-phase HPLC isocratic method that enables extraction, separation, identification, and quantification of all-trans-retinol and different molecular species of retinyl ester with high accuracy, sensitivity, and reliability.

Original languageEnglish (US)
Title of host publicationRetinoids
Subtitle of host publicationMethods and Protocols
Pages263-275
Number of pages13
DOIs
StatePublished - Dec 1 2010

Publication series

NameMethods in Molecular Biology
Volume652

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology

Keywords

  • Reverse-phase HPLC
  • mouse
  • quantification
  • retinoids
  • retinol
  • retinyl ester
  • separation
  • tissues
  • vitamin A

Fingerprint Dive into the research topics of 'Reverse-phase high-performance liquid chromatography (HPLC) analysis of retinol and retinyl esters in mouse serum and tissues'. Together they form a unique fingerprint.

  • Cite this