Rifampin and rpoB mutations can alter DNA supercoiling in Escherichia coli.

K. Drlica, R. J. Franco, T. R. Steck

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Two cases are described which indicate that RNA polymerase could alter DNA supercoiling. One occurred in a topA mutant in which abnormally high levels of plasmid supercoiling were lowered by rifampin, an inhibitor of the beta subunit of RNA polymerase. The second case involves suppression of a temperature-sensitive gyrB mutation by a rifampin-resistant allele of rpoB, the gene encoding the beta subunit of RNA polymerase. Measurements of chromosomal DNA supercoiling show that the rpoB mutation reduced DNA relaxation.

Original languageEnglish (US)
Pages (from-to)4983-4985
Number of pages3
JournalJournal of bacteriology
Volume170
Issue number10
DOIs
StatePublished - Oct 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Microbiology

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