RNA polymerase structure and function at lac operon

Sergei Borukhov, Jookyung Lee

Research output: Contribution to journalShort survey

8 Citations (Scopus)

Abstract

Transcription of E. coli lac operon by RNA polymerase (RNAP) is a classic example of how the basic functions of this enzyme, specifically the ability to recognize/bind promoters, melt the DNA and initiate RNA synthesis, is positively regulated by transcription activators, such as cyclic AMP-receptor protein, CRP, and negatively regulated by lac-repressor, LacI. In this review, we discuss the recent progress in structural and biochemical studies of RNAP and its binary and ternary complexes with CRP and lac promoter. With structural information now available for RNAP and models of binary and ternary elongation complexes, the interaction between these factors and RNAP can be modeled, and possible molecular mechanisms of their action can be inferred.

Original languageEnglish (US)
Pages (from-to)576-587
Number of pages12
JournalComptes Rendus - Biologies
Volume328
Issue number6 SPEC. ISS.
DOIs
StatePublished - Jan 1 2005

Fingerprint

Lac Operon
operon
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
Transcription
transcription (genetics)
Lac Repressors
promoter regions
Cyclic AMP Receptor Protein
cyclic AMP
Escherichia coli
Elongation
mechanism of action
RNA
receptors
synthesis
DNA
Enzymes
enzymes
proteins

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Borukhov, Sergei ; Lee, Jookyung. / RNA polymerase structure and function at lac operon. In: Comptes Rendus - Biologies. 2005 ; Vol. 328, No. 6 SPEC. ISS. pp. 576-587.
@article{7c57965147c74916b0f2d7a3c49529db,
title = "RNA polymerase structure and function at lac operon",
abstract = "Transcription of E. coli lac operon by RNA polymerase (RNAP) is a classic example of how the basic functions of this enzyme, specifically the ability to recognize/bind promoters, melt the DNA and initiate RNA synthesis, is positively regulated by transcription activators, such as cyclic AMP-receptor protein, CRP, and negatively regulated by lac-repressor, LacI. In this review, we discuss the recent progress in structural and biochemical studies of RNAP and its binary and ternary complexes with CRP and lac promoter. With structural information now available for RNAP and models of binary and ternary elongation complexes, the interaction between these factors and RNAP can be modeled, and possible molecular mechanisms of their action can be inferred.",
author = "Sergei Borukhov and Jookyung Lee",
year = "2005",
month = "1",
day = "1",
doi = "https://doi.org/10.1016/j.crvi.2005.03.007",
language = "English (US)",
volume = "328",
pages = "576--587",
journal = "Comptes Rendus - Biologies",
issn = "1631-0691",
publisher = "Elsevier Masson",
number = "6 SPEC. ISS.",

}

RNA polymerase structure and function at lac operon. / Borukhov, Sergei; Lee, Jookyung.

In: Comptes Rendus - Biologies, Vol. 328, No. 6 SPEC. ISS., 01.01.2005, p. 576-587.

Research output: Contribution to journalShort survey

TY - JOUR

T1 - RNA polymerase structure and function at lac operon

AU - Borukhov, Sergei

AU - Lee, Jookyung

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Transcription of E. coli lac operon by RNA polymerase (RNAP) is a classic example of how the basic functions of this enzyme, specifically the ability to recognize/bind promoters, melt the DNA and initiate RNA synthesis, is positively regulated by transcription activators, such as cyclic AMP-receptor protein, CRP, and negatively regulated by lac-repressor, LacI. In this review, we discuss the recent progress in structural and biochemical studies of RNAP and its binary and ternary complexes with CRP and lac promoter. With structural information now available for RNAP and models of binary and ternary elongation complexes, the interaction between these factors and RNAP can be modeled, and possible molecular mechanisms of their action can be inferred.

AB - Transcription of E. coli lac operon by RNA polymerase (RNAP) is a classic example of how the basic functions of this enzyme, specifically the ability to recognize/bind promoters, melt the DNA and initiate RNA synthesis, is positively regulated by transcription activators, such as cyclic AMP-receptor protein, CRP, and negatively regulated by lac-repressor, LacI. In this review, we discuss the recent progress in structural and biochemical studies of RNAP and its binary and ternary complexes with CRP and lac promoter. With structural information now available for RNAP and models of binary and ternary elongation complexes, the interaction between these factors and RNAP can be modeled, and possible molecular mechanisms of their action can be inferred.

UR - http://www.scopus.com/inward/record.url?scp=20444414890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444414890&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.crvi.2005.03.007

DO - https://doi.org/10.1016/j.crvi.2005.03.007

M3 - Short survey

C2 - 15950164

VL - 328

SP - 576

EP - 587

JO - Comptes Rendus - Biologies

JF - Comptes Rendus - Biologies

SN - 1631-0691

IS - 6 SPEC. ISS.

ER -