RNA polymerase structure and function at lac operon

Sergei Borukhov, Jookyung Lee

Research output: Contribution to journalShort survey

8 Scopus citations

Abstract

Transcription of E. coli lac operon by RNA polymerase (RNAP) is a classic example of how the basic functions of this enzyme, specifically the ability to recognize/bind promoters, melt the DNA and initiate RNA synthesis, is positively regulated by transcription activators, such as cyclic AMP-receptor protein, CRP, and negatively regulated by lac-repressor, LacI. In this review, we discuss the recent progress in structural and biochemical studies of RNAP and its binary and ternary complexes with CRP and lac promoter. With structural information now available for RNAP and models of binary and ternary elongation complexes, the interaction between these factors and RNAP can be modeled, and possible molecular mechanisms of their action can be inferred.

Original languageEnglish (US)
Pages (from-to)576-587
Number of pages12
JournalComptes Rendus - Biologies
Volume328
Issue number6 SPEC. ISS.
DOIs
StatePublished - Jun 2005

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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