φ6 is a bacteriophage containing three pieces of double-stranded RNA (dsRNA) in its virion (Semancik et al., 1973). The synthesis of viral single-stranded RNA (ssRNA) and dsRNA was studied in cells infected by nonsense mutants of bacteriophage φ6. Eight classes of mutants, lacking 9 of the 12 viral proteins, were tested, and it was found that two classes designated 1 and 2 were defective in the synthesis of both dsRNA and the medium (m) and small (s) ssRNA species. It is concluded that proteins P1 and P2, which are found in the core of the virion, are components of either a transcriptase or a replicase or both.
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