ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles

Yoshihiko Shimizu, Dean Thumkeo, Jeongsin Keel, Toshimasa Ishizaki, Hiroko Oshima, Masanobu Oshima, Yoichi Noda, Fumio Matsumura, Makoto M. Taketo, Shuh Narumiya

Research output: Contribution to journalArticlepeer-review

237 Scopus citations

Abstract

Rho-associated kinase (ROCK) I mediates signaling from Rho to the actin cytoskeleton. To investigate the in vivo functions of ROCK-I, we generated ROCK-I-deficient mice. Loss of ROCK-I resulted in failure of eyelid closure and closure of the ventral body wall, which gave rise to the eyes open at birth and omphalocele phenotypes in neonates. Most ROCK-I-/- mice died soon after birth as a result of cannibalization of the omphalocele by the mother. Actin cables that encircle the eye in the epithelial cells of the eyelid were disorganized and accumulation of filamentous actin at the umbilical ring was impaired, with loss of phosphorylation of the myosin regulatory light chain (MLC) at both sites, in ROCK-I-/- embryos. Stress fiber formation and MLC phosphorylation induced by EGF were also attenuated in primary keratinocytes from ROCK-I-/- mice. These results suggest that ROCK-I regulates closure of the eyelids and ventral body wall through organization of actomyosin bundles.

Original languageEnglish (US)
Pages (from-to)941-953
Number of pages13
JournalJournal of Cell Biology
Volume168
Issue number6
DOIs
StatePublished - Mar 2005

ASJC Scopus subject areas

  • Cell Biology

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