Role of β-endorphin, corticotropin-releasing hormone, and autonomic nervous system in mediation of the effect of chronic ethanol on natural killer cell cytolytic activity

Nadka Boyadjieva, Juan Advis, Dipak Sarkar

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: We have recently shown that alcohol feeding suppresses natural killer (NK) cell cytolytic activity partly by decreasing the function of hypothalamic β-endorphin (β-EP) neurons. The neuronal mechanism by which hypothalamic β-EP communicates with the spleen to regulate the action of ethanol on NK cells is not known. In the present study, we evaluated the roles of β-EP neurons, corticotropin releasing hormone (CRH) neurons, and the autonomic nervous system (ANS) in regulation of the ethanol effect on splenic NK cell cytolytic function. Methods: Male rats were fed an ethanol-containing liquid diet or control diets. These rats were used to determine the hormone release from the paraventricular nuclei (PVN) of the hypothalamus or used to determine the splenic NK cell cytolytic function after PVN administration of CRH or intraperitoneal (i.p.) administration of a ganglionic blocker chlorisondamine. The release of hormones from the PVN was measured using the push-pull perfusion method. Splenic cytolytic activity was determined using the 4-hour 51Cr release assay against YAC-1 lymphoma target cells. Results: Alcohol feeding decreased the amount of β-EP but increased the amount of CRH in the push-pull perfusate (PPP) samples collected from the PVN. When exogenous β-EP was perfused into the PVN, it suppressed the release of endogenous CRH found in PPP samples of the PVN. Conversely, perfusion of an opiate antagonist naltrexone into the PVN increased the levels of endogenous CRH in PPP samples of the PVN. In addition, administration of exogenous β-EP in the PVN stimulated the cytolytic function of NK cells, an action that was antagonized by CRH as well as by ethanol. Corticotropin- releasing hormone and ethanol alone also had an inhibitory action on NK cells. Finally, the ganglionic blocker used prevented the effect that ethanol, β-EP, and CRH had on NK cells. These data suggest that ethanol inhibits the function of NK cells partly by suppressing the influence of the β-EP-CRH-ANS signal to the spleen.

Original languageEnglish (US)
Pages (from-to)1761-1767
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume30
Issue number10
DOIs
StatePublished - Oct 1 2006

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Endorphins
Paraventricular Hypothalamic Nucleus
Corticotropin-Releasing Hormone
Autonomic Nervous System
Neurology
Natural Killer Cells
Ethanol
Ganglionic Blockers
Neurons
Nutrition
Rats
Opiate Alkaloids
Spleen
Chlorisondamine
Perfusion
Alcohols
Hormones
Diet
Naltrexone
Hypothalamus

All Science Journal Classification (ASJC) codes

  • Psychiatry and Mental health
  • Medicine (miscellaneous)
  • Toxicology

Keywords

  • Autonomic Nervous System
  • Corticotropin-Releasing Hormone
  • Ganglionic Blocker
  • Natural Killer Cells
  • Rats
  • β-Endorphin

Cite this

@article{37c8fa1aa05d44098b1cb51bbfc7e0d7,
title = "Role of β-endorphin, corticotropin-releasing hormone, and autonomic nervous system in mediation of the effect of chronic ethanol on natural killer cell cytolytic activity",
abstract = "Background: We have recently shown that alcohol feeding suppresses natural killer (NK) cell cytolytic activity partly by decreasing the function of hypothalamic β-endorphin (β-EP) neurons. The neuronal mechanism by which hypothalamic β-EP communicates with the spleen to regulate the action of ethanol on NK cells is not known. In the present study, we evaluated the roles of β-EP neurons, corticotropin releasing hormone (CRH) neurons, and the autonomic nervous system (ANS) in regulation of the ethanol effect on splenic NK cell cytolytic function. Methods: Male rats were fed an ethanol-containing liquid diet or control diets. These rats were used to determine the hormone release from the paraventricular nuclei (PVN) of the hypothalamus or used to determine the splenic NK cell cytolytic function after PVN administration of CRH or intraperitoneal (i.p.) administration of a ganglionic blocker chlorisondamine. The release of hormones from the PVN was measured using the push-pull perfusion method. Splenic cytolytic activity was determined using the 4-hour 51Cr release assay against YAC-1 lymphoma target cells. Results: Alcohol feeding decreased the amount of β-EP but increased the amount of CRH in the push-pull perfusate (PPP) samples collected from the PVN. When exogenous β-EP was perfused into the PVN, it suppressed the release of endogenous CRH found in PPP samples of the PVN. Conversely, perfusion of an opiate antagonist naltrexone into the PVN increased the levels of endogenous CRH in PPP samples of the PVN. In addition, administration of exogenous β-EP in the PVN stimulated the cytolytic function of NK cells, an action that was antagonized by CRH as well as by ethanol. Corticotropin- releasing hormone and ethanol alone also had an inhibitory action on NK cells. Finally, the ganglionic blocker used prevented the effect that ethanol, β-EP, and CRH had on NK cells. These data suggest that ethanol inhibits the function of NK cells partly by suppressing the influence of the β-EP-CRH-ANS signal to the spleen.",
keywords = "Autonomic Nervous System, Corticotropin-Releasing Hormone, Ganglionic Blocker, Natural Killer Cells, Rats, β-Endorphin",
author = "Nadka Boyadjieva and Juan Advis and Dipak Sarkar",
year = "2006",
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TY - JOUR

T1 - Role of β-endorphin, corticotropin-releasing hormone, and autonomic nervous system in mediation of the effect of chronic ethanol on natural killer cell cytolytic activity

AU - Boyadjieva, Nadka

AU - Advis, Juan

AU - Sarkar, Dipak

PY - 2006/10/1

Y1 - 2006/10/1

N2 - Background: We have recently shown that alcohol feeding suppresses natural killer (NK) cell cytolytic activity partly by decreasing the function of hypothalamic β-endorphin (β-EP) neurons. The neuronal mechanism by which hypothalamic β-EP communicates with the spleen to regulate the action of ethanol on NK cells is not known. In the present study, we evaluated the roles of β-EP neurons, corticotropin releasing hormone (CRH) neurons, and the autonomic nervous system (ANS) in regulation of the ethanol effect on splenic NK cell cytolytic function. Methods: Male rats were fed an ethanol-containing liquid diet or control diets. These rats were used to determine the hormone release from the paraventricular nuclei (PVN) of the hypothalamus or used to determine the splenic NK cell cytolytic function after PVN administration of CRH or intraperitoneal (i.p.) administration of a ganglionic blocker chlorisondamine. The release of hormones from the PVN was measured using the push-pull perfusion method. Splenic cytolytic activity was determined using the 4-hour 51Cr release assay against YAC-1 lymphoma target cells. Results: Alcohol feeding decreased the amount of β-EP but increased the amount of CRH in the push-pull perfusate (PPP) samples collected from the PVN. When exogenous β-EP was perfused into the PVN, it suppressed the release of endogenous CRH found in PPP samples of the PVN. Conversely, perfusion of an opiate antagonist naltrexone into the PVN increased the levels of endogenous CRH in PPP samples of the PVN. In addition, administration of exogenous β-EP in the PVN stimulated the cytolytic function of NK cells, an action that was antagonized by CRH as well as by ethanol. Corticotropin- releasing hormone and ethanol alone also had an inhibitory action on NK cells. Finally, the ganglionic blocker used prevented the effect that ethanol, β-EP, and CRH had on NK cells. These data suggest that ethanol inhibits the function of NK cells partly by suppressing the influence of the β-EP-CRH-ANS signal to the spleen.

AB - Background: We have recently shown that alcohol feeding suppresses natural killer (NK) cell cytolytic activity partly by decreasing the function of hypothalamic β-endorphin (β-EP) neurons. The neuronal mechanism by which hypothalamic β-EP communicates with the spleen to regulate the action of ethanol on NK cells is not known. In the present study, we evaluated the roles of β-EP neurons, corticotropin releasing hormone (CRH) neurons, and the autonomic nervous system (ANS) in regulation of the ethanol effect on splenic NK cell cytolytic function. Methods: Male rats were fed an ethanol-containing liquid diet or control diets. These rats were used to determine the hormone release from the paraventricular nuclei (PVN) of the hypothalamus or used to determine the splenic NK cell cytolytic function after PVN administration of CRH or intraperitoneal (i.p.) administration of a ganglionic blocker chlorisondamine. The release of hormones from the PVN was measured using the push-pull perfusion method. Splenic cytolytic activity was determined using the 4-hour 51Cr release assay against YAC-1 lymphoma target cells. Results: Alcohol feeding decreased the amount of β-EP but increased the amount of CRH in the push-pull perfusate (PPP) samples collected from the PVN. When exogenous β-EP was perfused into the PVN, it suppressed the release of endogenous CRH found in PPP samples of the PVN. Conversely, perfusion of an opiate antagonist naltrexone into the PVN increased the levels of endogenous CRH in PPP samples of the PVN. In addition, administration of exogenous β-EP in the PVN stimulated the cytolytic function of NK cells, an action that was antagonized by CRH as well as by ethanol. Corticotropin- releasing hormone and ethanol alone also had an inhibitory action on NK cells. Finally, the ganglionic blocker used prevented the effect that ethanol, β-EP, and CRH had on NK cells. These data suggest that ethanol inhibits the function of NK cells partly by suppressing the influence of the β-EP-CRH-ANS signal to the spleen.

KW - Autonomic Nervous System

KW - Corticotropin-Releasing Hormone

KW - Ganglionic Blocker

KW - Natural Killer Cells

KW - Rats

KW - β-Endorphin

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U2 - https://doi.org/10.1111/j.1530-0277.2006.00209.x

DO - https://doi.org/10.1111/j.1530-0277.2006.00209.x

M3 - Article

C2 - 17010143

VL - 30

SP - 1761

EP - 1767

JO - Alcoholism: Clinical and Experimental Research

JF - Alcoholism: Clinical and Experimental Research

SN - 0145-6008

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ER -