Role of adrenergic and cholinergic mediators in salivary phospholipids secretion

Bronislaw L. Slomiany, Shipra Sengupta, Elzbieta Piotrowski, Ramon A. Lopez, Amalia Slomiany

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The influence of adrenergic and cholinergic mediators on phospholipid secretion in rat sublingual salivary gland cells maintained in the presence of [3H]choline was investigated. The secretion of [3H]choline-containing phospholipids over 30 min period averaged 1.93% of the total cellular labeled phospholipids in the absence of any mediator, and was enhanced by β-adrenergic agonist, isoproterenol, to a greater extent than the cholinergic agonists, pilocarpine and carbachol. A 2.9-fold increase in phospholipid secretion occurred with isoproterenol, while pilocarpine and carbachol evoked only 1.3-fold increase. The effect of isoproterenol was inhibited by alprenolol and that of pilocarpine and carbachol by atropine. In contrast to pilocarpine and carbachol, the enhanced phospholipid secretion due to isoproterenol was accompanied by an increase in cAMP concentration. The secretion of phospholipids was also stimulated by dibutyryl-cAMP and the protein kinase C activator, phorbol myristate acetate, but not by 4α-phorbol 12,13-didecanoate which does not activate protein kinase C. Furthermore, the effects of dibutyrylcAMP and phorbol myristate acetate were additive. The phospholipids secreted in response to isoproterenol exhibited a 52% decrease in lysophosphatidylcholine, while those secreted in response to pilocarpine and carbachol showed a 21-23% lower content of phosphatidylcholine, and were enriched in lysophosphatidylcholine (2.6-2.8-fold) and sphingomyelin (1.5-1.6-fold). The results indicate that salivary phospholipid secretion remains mainly under β-adrenergic regulation, while the phospholipid makeup of the secretion is under cholinergic control.

Original languageAmerican English
Pages (from-to)171-177
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Issue number2
StatePublished - Mar 4 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology


  • Adrenergic
  • Cholinergic mediation
  • Salivary phospholipid
  • Secretion


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