Rosmanol potently induces apoptosis through both the mitochondrial apoptotic pathway and death receptor pathway in human colon adenocarcinoma COLO 205 cells

An Chin Cheng, Ming Fen Lee, Mei Ling Tsai, Ching Shu Lai, Jong Hun Lee, Chi-Tang Ho, Min Hsiung Pan

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Rosemary (Rosmarinus officinalis), a culinary spice and medicinal herb, has been widely used in European folk medicine to treat numerous ailments. Many studies have shown that rosemary extracts play important roles in anti-inflammation, anti-tumor, and anti-proliferation in various in vitro and in vivo settings. The roles of tumor suppression of rosemary have been attributed to the major components, including carnosic acid, carnosol, and rosmarinic acid, rosmanol, and ursolic acid. This study was to explore the effect of rosmanol on the growth of COLO 205 human colorectal adenocarcinoma cells and to delineate the underlying mechanisms. When treated with 50μM of rosmanol for 24h, COLO 205 cells displayed a strong apoptosis-inducing response with a 51% apoptotic ratio (IC50 ∼42μM). Rosmanol increased the expression of Fas and FasL, led to the cleavage and activation of pro-caspase-8 and Bid, and mobilized Bax from cytosol into mitochondria. The mutual activation between tBid and Bad decreased the mitochondrial membrane potential and released cytochrome c and apoptosis-inducing factor (AIF) to cytosol. In turn, cytochrome c induced the processing of pro-caspase-9 and pro-caspase-3, followed by the cleavage of poly-(ADP-ribose) polymerase (PARP) and DNA fragmentation factor (DFF-45). These results demonstrate that the rosmanol-induced apoptosis in COLO 205 cells is involvement of caspase activation and involving complicated regulation of both the mitochondrial apoptotic pathway and death receptor pathway.

Original languageEnglish (US)
Pages (from-to)485-493
Number of pages9
JournalFood and Chemical Toxicology
Volume49
Issue number2
DOIs
StatePublished - Feb 1 2011

Fingerprint

Death Domain Receptors
rosemary
adenocarcinoma
colon
Colon
Adenocarcinoma
apoptosis
cytochrome c
Apoptosis
cytosol
Chemical activation
caspase-8
caspase-9
ursolic acid
NAD ADP-ribosyltransferase
rosmarinic acid
Rosmarinus officinalis
Cytochromes c
neoplasms
Cytosol

All Science Journal Classification (ASJC) codes

  • Food Science
  • Toxicology

Keywords

  • Apoptosis
  • COLO 205 human colon adenocarcinoma cells
  • Death receptor pathway
  • Mitochondrial apoptotic pathway
  • Rosemary
  • Rosmanol

Cite this

Cheng, An Chin ; Lee, Ming Fen ; Tsai, Mei Ling ; Lai, Ching Shu ; Lee, Jong Hun ; Ho, Chi-Tang ; Pan, Min Hsiung. / Rosmanol potently induces apoptosis through both the mitochondrial apoptotic pathway and death receptor pathway in human colon adenocarcinoma COLO 205 cells. In: Food and Chemical Toxicology. 2011 ; Vol. 49, No. 2. pp. 485-493.
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abstract = "Rosemary (Rosmarinus officinalis), a culinary spice and medicinal herb, has been widely used in European folk medicine to treat numerous ailments. Many studies have shown that rosemary extracts play important roles in anti-inflammation, anti-tumor, and anti-proliferation in various in vitro and in vivo settings. The roles of tumor suppression of rosemary have been attributed to the major components, including carnosic acid, carnosol, and rosmarinic acid, rosmanol, and ursolic acid. This study was to explore the effect of rosmanol on the growth of COLO 205 human colorectal adenocarcinoma cells and to delineate the underlying mechanisms. When treated with 50μM of rosmanol for 24h, COLO 205 cells displayed a strong apoptosis-inducing response with a 51{\%} apoptotic ratio (IC50 ∼42μM). Rosmanol increased the expression of Fas and FasL, led to the cleavage and activation of pro-caspase-8 and Bid, and mobilized Bax from cytosol into mitochondria. The mutual activation between tBid and Bad decreased the mitochondrial membrane potential and released cytochrome c and apoptosis-inducing factor (AIF) to cytosol. In turn, cytochrome c induced the processing of pro-caspase-9 and pro-caspase-3, followed by the cleavage of poly-(ADP-ribose) polymerase (PARP) and DNA fragmentation factor (DFF-45). These results demonstrate that the rosmanol-induced apoptosis in COLO 205 cells is involvement of caspase activation and involving complicated regulation of both the mitochondrial apoptotic pathway and death receptor pathway.",
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Rosmanol potently induces apoptosis through both the mitochondrial apoptotic pathway and death receptor pathway in human colon adenocarcinoma COLO 205 cells. / Cheng, An Chin; Lee, Ming Fen; Tsai, Mei Ling; Lai, Ching Shu; Lee, Jong Hun; Ho, Chi-Tang; Pan, Min Hsiung.

In: Food and Chemical Toxicology, Vol. 49, No. 2, 01.02.2011, p. 485-493.

Research output: Contribution to journalArticle

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