S-phase Enriched Non-coding RNAs Regulate Gene Expression and Cell Cycle Progression

Ozlem Yildirim, Enver C. Izgu, Manashree Damle, Vladislava Chalei, Fei Ji, Ruslan I. Sadreyev, Jack W. Szostak, Robert E. Kingston

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Many proteins that are needed for progression through S-phase are produced from transcripts that peak in the S-phase, linking temporal expression of those proteins to the time that they are required in cell cycle. Here, we explore the potential roles of long non-coding RNAs in cell cycle progression. We use a sensitive click-chemistry approach to isolate nascent RNAs in a human cell line, and we identify more than 900 long non-coding RNAs (lncRNAs) whose synthesis peaks during the S-phase. More than 200 of these are long intergenic non-coding RNAs (lincRNAs) with S-phase-specific expression. We characterize three of these lincRNAs by knockdown and find that all three lincRNAs are required for appropriate S-phase progression. We infer that non-coding RNAs are key regulatory effectors during the cell cycle, acting on distinct regulatory networks, and herein, we provide a large catalog of candidate cell-cycle regulatory RNAs.

Original languageEnglish (US)
Article number107629
JournalCell Reports
Volume31
Issue number6
DOIs
StatePublished - May 12 2020

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • S-phase
  • cell cycle
  • click chemistry
  • lincRNA
  • metabolic labeling
  • nascent RNA
  • non-coding RNA

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