Scope and predictive genetic/phenotypic signatures of bicarbonate (NaHCO3) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus

Selvi C. Ersoy; Mariam Otmishi; Vanessa T. Milan; Liang Li; Youngju Pak; Jose Mediavilla; Liang Chen; Barry Kreiswirth; Henry F. Chambers; Richard A. Proctor; Yan Q. Xiong; Vance G. Fowler; Arnold S. Bayer

doi:10.1128/AAC.02445-19

Scope and predictive genetic/phenotypic signatures of bicarbonate (NaHCO₃) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus

Selvi C. Ersoy
, Mariam Otmishi
, Vanessa T. Milan
, Liang Li
, Youngju Pak
, Jose Mediavilla
, Liang Chen
, Barry Kreiswirth
, Henry F. Chambers
, Richard A. Proctor
, Yan Q. Xiong
, Vance G. Fowler
, Arnold S. Bayer

Hackensack Meridian School of Medicine, Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

Abstract

Addition of sodium bicarbonate (NaHCO₃) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO₃ responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO₃ responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO₃ responsiveness for OXA.

Original language	English
Article number	e02445-19
Journal	Antimicrobial Agents and Chemotherapy
Volume	64
Issue number	5
DOIs	https://doi.org/10.1128/AAC.02445-19
State	Published - May 1 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

Keywords

Antimicrobial susceptibility testing
Beta-lactams
Methicillin-resistant Staphylococcus aureus
Sodium bicarbonate

Access to Document

10.1128/AAC.02445-19

Cite this

Ersoy, S. C., Otmishi, M., Milan, V. T., Li, L., Pak, Y., Mediavilla, J., Chen, L., Kreiswirth, B., Chambers, H. F., Proctor, R. A., Xiong, Y. Q., Fowler, V. G., & Bayer, A. S. (2020). Scope and predictive genetic/phenotypic signatures of bicarbonate (NaHCO₃) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 64(5), Article e02445-19. https://doi.org/10.1128/AAC.02445-19

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title = "Scope and predictive genetic/phenotypic signatures of bicarbonate (NaHCO3) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus",

abstract = "Addition of sodium bicarbonate (NaHCO3) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO3 responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75\% and ∼36\% of isolates displayed the NaHCO3 responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO3 responsiveness for OXA.",

keywords = "Antimicrobial susceptibility testing, Beta-lactams, Methicillin-resistant Staphylococcus aureus, Sodium bicarbonate",

author = "Ersoy, \{Selvi C.\} and Mariam Otmishi and Milan, \{Vanessa T.\} and Liang Li and Youngju Pak and Jose Mediavilla and Liang Chen and Barry Kreiswirth and Chambers, \{Henry F.\} and Proctor, \{Richard A.\} and Xiong, \{Yan Q.\} and Fowler, \{Vance G.\} and Bayer, \{Arnold S.\}",

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year = "2020",

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doi = "10.1128/AAC.02445-19",

language = "English",

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Ersoy, SC, Otmishi, M, Milan, VT, Li, L, Pak, Y, Mediavilla, J, Chen, L, Kreiswirth, B, Chambers, HF, Proctor, RA, Xiong, YQ, Fowler, VG & Bayer, AS 2020, 'Scope and predictive genetic/phenotypic signatures of bicarbonate (NaHCO₃) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus', Antimicrobial Agents and Chemotherapy, vol. 64, no. 5, e02445-19. https://doi.org/10.1128/AAC.02445-19

Scope and predictive genetic/phenotypic signatures of bicarbonate (NaHCO₃) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus. / Ersoy, Selvi C.; Otmishi, Mariam; Milan, Vanessa T. et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 64, No. 5, e02445-19, 01.05.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Scope and predictive genetic/phenotypic signatures of bicarbonate (NaHCO3) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus

AU - Ersoy, Selvi C.

AU - Otmishi, Mariam

AU - Milan, Vanessa T.

AU - Li, Liang

AU - Pak, Youngju

AU - Mediavilla, Jose

AU - Chen, Liang

AU - Kreiswirth, Barry

AU - Chambers, Henry F.

AU - Proctor, Richard A.

AU - Xiong, Yan Q.

AU - Fowler, Vance G.

AU - Bayer, Arnold S.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Addition of sodium bicarbonate (NaHCO3) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO3 responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO3 responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO3 responsiveness for OXA.

AB - Addition of sodium bicarbonate (NaHCO3) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO3 responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO3 responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO3 responsiveness for OXA.

KW - Antimicrobial susceptibility testing

KW - Beta-lactams

KW - Methicillin-resistant Staphylococcus aureus

KW - Sodium bicarbonate

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