Secreted factors from bone marrow stromal cells upregulate IL-10 and reverse acute kidney injury

Jack M. Milwid, Takaharu Ichimura, Matthew Li, Yunxin Jiao, Jungwoo Lee, Joshua S. Yarmush, Biju Parekkadan, Arno W. Tilles, Joseph V. Bonventre, Martin L. Yarmush

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Acute kidney injury is a devastating syndrome that afflicts over 2,000,000 people in the US per year, with an associated mortality of greater than 70% in severe cases. Unfortunately, standard-of-care treatments are not sufficient for modifying the course of disease. Many groups have explored the use of bone marrow stromal cells (BMSCs) for the treatment of AKI because BMSCs have been shown to possess unique anti-inflammatory, cytoprotective, and regenerative properties in vitro and in vivo. It is yet unresolved whether the primary mechanisms controlling BMSC therapy in AKI depend on direct cell infusion, or whether BMSC-secreted factors alone are sufficient for mitigating the injury. Here we show that BMSC-secreted factors are capable of providing a survival benefit to rats subjected to cisplatin-induced AKI. We observed that when BMSC-conditioned medium (BMSC-CM) is administered intravenously, it prevents tubular apoptosis and necrosis and ameliorates AKI. In addition, we observed that BMSC-CM causes IL-10 upregulation in treated animals, which is important to animal survival and protection of the kidney. In all, these results demonstrate that BMSC-secreted factors are capable of providing support without cell transplantation, and the IL-10 increase seen in BMSC-CM-treated animals correlates with attenuation of severe AKI.

Original languageEnglish (US)
Article number392050
JournalStem Cells International
DOIs
StatePublished - 2012

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Secreted factors from bone marrow stromal cells upregulate IL-10 and reverse acute kidney injury'. Together they form a unique fingerprint.

  • Cite this

    Milwid, J. M., Ichimura, T., Li, M., Jiao, Y., Lee, J., Yarmush, J. S., Parekkadan, B., Tilles, A. W., Bonventre, J. V., & Yarmush, M. L. (2012). Secreted factors from bone marrow stromal cells upregulate IL-10 and reverse acute kidney injury. Stem Cells International, [392050]. https://doi.org/10.1155/2012/392050