Self-assembling multidomain peptides tailor biological responses through biphasic release

Vivek Kumar, Nichole L. Taylor, Siyu Shi, Navindee C. Wickremasinghe, Rena N. D'Souza, Jeffrey D. Hartgerink

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Delivery of small molecules and drugs to tissues is a mainstay of several tissue engineering strategies. Next generation treatments focused on localized drug delivery offer a more effective means in dealing with refractory healing when compared to systemic approaches. Here we describe a novel multidomain peptide hydrogel that capitalizes on synthetic peptide chemistry, supramolecular self-assembly and cytokine delivery to tailor biological responses. This material is biomimetic, shows shear stress recovery and offers a nanofibrous matrix that sequesters cytokines. The biphasic pattern of cytokine release results in the spatio-temporal activation of THP-1 monocytes and macrophages. Furthermore, macrophage-material interactions are promoted without generation of a proinflammatory environment. Subcutaneous implantation of injectable scaffolds showed a marked increase in macrophage infiltration and polarization dictated by cytokine loading as early as 3 days, with complete scaffold resorption by day 14. Macrophage interaction and response to the peptide composite facilitated the (i) recruitment of monocytes/macrophages, (ii) sustained residence of immune cells until degradation, and (iii) promotion of a pro-resolution M2 environment. Our results suggest the potential use of this injectable cytokine loaded hydrogel scaffold in a variety of tissue engineering applications.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalBiomaterials
Volume52
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Macrophages
Peptides
Cytokines
Scaffolds (biology)
Hydrogel
Tissue Engineering
Tissue engineering
Hydrogels
Monocytes
Biomimetic Materials
Biomimetic materials
Supramolecular chemistry
Injections
Drug delivery
Infiltration
Pharmaceutical Preparations
Refractory materials
Self assembly
Shear stress
Chemical activation

All Science Journal Classification (ASJC) codes

  • Mechanics of Materials
  • Ceramics and Composites
  • Bioengineering
  • Biophysics
  • Biomaterials

Keywords

  • Inflammation
  • Macrophage polarization
  • Multi-domain peptide
  • Self-assembly

Cite this

Kumar, V., Taylor, N. L., Shi, S., Wickremasinghe, N. C., D'Souza, R. N., & Hartgerink, J. D. (2015). Self-assembling multidomain peptides tailor biological responses through biphasic release. Biomaterials, 52(1), 71-78. https://doi.org/10.1016/j.biomaterials.2015.01.079
Kumar, Vivek ; Taylor, Nichole L. ; Shi, Siyu ; Wickremasinghe, Navindee C. ; D'Souza, Rena N. ; Hartgerink, Jeffrey D. / Self-assembling multidomain peptides tailor biological responses through biphasic release. In: Biomaterials. 2015 ; Vol. 52, No. 1. pp. 71-78.
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Kumar, V, Taylor, NL, Shi, S, Wickremasinghe, NC, D'Souza, RN & Hartgerink, JD 2015, 'Self-assembling multidomain peptides tailor biological responses through biphasic release', Biomaterials, vol. 52, no. 1, pp. 71-78. https://doi.org/10.1016/j.biomaterials.2015.01.079

Self-assembling multidomain peptides tailor biological responses through biphasic release. / Kumar, Vivek; Taylor, Nichole L.; Shi, Siyu; Wickremasinghe, Navindee C.; D'Souza, Rena N.; Hartgerink, Jeffrey D.

In: Biomaterials, Vol. 52, No. 1, 01.01.2015, p. 71-78.

Research output: Contribution to journalArticle

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