Self-assembly of DNA parallel double-crossover motifs

Jung Yeon Lee, Qi Yang, Xu Chang, Maciej Jeziorek, Devanathan Perumal, Tiffany R. Olivera, Jean Pierre Etchegaray, Fei Zhang

Research output: Contribution to journalArticlepeer-review


DNA double-crossover motifs, including parallel and antiparallel crossovers, serve as the structural foundation for the creation of diverse nanostructures and dynamic devices in DNA nanotechnology. Parallel crossover motifs have unique advantages over the widely used antiparallel crossover design but have not developed as substantially due to the difficulties in assembly. Here we created 29 designs of parallel double-crossover motifs varying in hybridization pathways, central domain lengths, and crossover locations to investigate their assembly mechanism. Arrays were successfully formed in four distinct designs, and large tubular structures were obtained in seven designs with predefined pathways and central domains appoximately 16 nucleotides in length. The nanotubes obtained from parallel crossover design showed improved nuclease resistance than the ones from the antiparallel counterpart design. Overall, our study provides a basis for the development of generalized assembly rules of DNA parallel crossover systems and opens new opportunities for their potential use in biological systems.

Original languageAmerican English
Pages (from-to)1685-1691
Number of pages7
Issue number4
StatePublished - Jan 8 2024

ASJC Scopus subject areas

  • General Materials Science

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