Sensitivity-Enhanced Sim-CT HMQC PFG-HBHA(CO)NH and PFG-CBCA(CO)NH Triple-Resonance Experiments

G. V.T. Swapna, Gaetano T. Montelione

Research output: Contribution to journalEditorialpeer-review

1 Scopus citations

Abstract

Short transverse relaxation times of C α and C β single-quantum coherences reduce the sensitivity of triple-resonance experiments involving transfers of C α /C β or H α /H β coherences. Multiple-quantum line-narrowing techniques improve the relaxation properties of 13 C coherences, thereby increasing the sensitivity of the experiment. In the present work, we describe PFG-CBCA(CO)NH and PFG-HBHA(CO)NH experiments that utilize heteronuclear multiple-quantum coherences in a simultaneous constant-time period to obtain completely decoupled spectra with improved sensitivity. Results indicate that ∼30% of cross peaks show an average enhancement of ∼15% in the CBCA(CO)NH experiment. In the related HBHA(CO)NH experiment, ∼97% of the cross peaks show an average enhancement of ∼40%.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalJournal of Magnetic Resonance
Volume137
Issue number2
DOIs
StatePublished - Apr 1999

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Keywords

  • NMR resonance assignments
  • Nuclear relaxation
  • Protein NMR
  • Simultaneous constant-time period

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