Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane

H. Wang, D. S. Leonard, A. A. Castellarin, I. Tsukahara, Y. Ninomiya, F. Yagi, N. Cheewatrakoolpong, I. K. Sugino, Marco Zarbin

Research output: Contribution to journalArticle

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Abstract

PURPOSE. To investigate the survival and behavior of retinal pigment epithelium (RPE) microaggregates transplanted onto hydraulically debrided Bruch's membrane and to compare results of using three different vehicles for cell delivery. METHODS. RPE microaggregates obtained from male cats were transplanted onto the tapetal area of female cats after native RPE was debrided. For the control, one of three vehicles was introduced into the debridements. Each transplant or control specimen was analyzed histologically and immunohistochemically. Transplanted male RPE cells were identified by in situ labeling of the cat Y chromosome. RESULTS. Histologically, significant numbers of condensed, darkly stained RPE nuclei were observed in all transplants compared with few TUNEL-positive RPE cells. Cellular retinal-dehyde-binding protein was present up to day 7 in all RPE cells in transplants. In both transplant and control specimens, the antibody against the Ki-67 nuclear antigen labeled some RPE cells at day 3. TUNEL-positive outer nuclear layer nuclei were most frequently observed at day 1, but were much less frequent at 7 days in both transplant and control specimens. CONCLUSIONS. Transplanted RPE appeared to retain at least some markers of differentiation up to 7 days after surgery. Some proliferation of transplanted RPE cells was also seen. Apoptotic cell death of transplanted RPE, as judged by TUNEL staining was observed rarely. RPE transplants imposed no adverse effect on the overlying retina. RPE survival appeared to be similar with each of the three vehicles for cell delivery.

Original languageEnglish (US)
Pages (from-to)2990-2999
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume42
Issue number12
StatePublished - Nov 17 2001

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Bruch Membrane
Retinal Pigment Epithelium
Transplants
In Situ Nick-End Labeling
Cats
Ki-67 Antigen
Nuclear Antigens
Y Chromosome
Differentiation Antigens
Debridement
Ambulatory Surgical Procedures

All Science Journal Classification (ASJC) codes

  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Ophthalmology

Cite this

Wang, H., Leonard, D. S., Castellarin, A. A., Tsukahara, I., Ninomiya, Y., Yagi, F., ... Zarbin, M. (2001). Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane. Investigative Ophthalmology and Visual Science, 42(12), 2990-2999.
Wang, H. ; Leonard, D. S. ; Castellarin, A. A. ; Tsukahara, I. ; Ninomiya, Y. ; Yagi, F. ; Cheewatrakoolpong, N. ; Sugino, I. K. ; Zarbin, Marco. / Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane. In: Investigative Ophthalmology and Visual Science. 2001 ; Vol. 42, No. 12. pp. 2990-2999.
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abstract = "PURPOSE. To investigate the survival and behavior of retinal pigment epithelium (RPE) microaggregates transplanted onto hydraulically debrided Bruch's membrane and to compare results of using three different vehicles for cell delivery. METHODS. RPE microaggregates obtained from male cats were transplanted onto the tapetal area of female cats after native RPE was debrided. For the control, one of three vehicles was introduced into the debridements. Each transplant or control specimen was analyzed histologically and immunohistochemically. Transplanted male RPE cells were identified by in situ labeling of the cat Y chromosome. RESULTS. Histologically, significant numbers of condensed, darkly stained RPE nuclei were observed in all transplants compared with few TUNEL-positive RPE cells. Cellular retinal-dehyde-binding protein was present up to day 7 in all RPE cells in transplants. In both transplant and control specimens, the antibody against the Ki-67 nuclear antigen labeled some RPE cells at day 3. TUNEL-positive outer nuclear layer nuclei were most frequently observed at day 1, but were much less frequent at 7 days in both transplant and control specimens. CONCLUSIONS. Transplanted RPE appeared to retain at least some markers of differentiation up to 7 days after surgery. Some proliferation of transplanted RPE cells was also seen. Apoptotic cell death of transplanted RPE, as judged by TUNEL staining was observed rarely. RPE transplants imposed no adverse effect on the overlying retina. RPE survival appeared to be similar with each of the three vehicles for cell delivery.",
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Wang, H, Leonard, DS, Castellarin, AA, Tsukahara, I, Ninomiya, Y, Yagi, F, Cheewatrakoolpong, N, Sugino, IK & Zarbin, M 2001, 'Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane', Investigative Ophthalmology and Visual Science, vol. 42, no. 12, pp. 2990-2999.

Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane. / Wang, H.; Leonard, D. S.; Castellarin, A. A.; Tsukahara, I.; Ninomiya, Y.; Yagi, F.; Cheewatrakoolpong, N.; Sugino, I. K.; Zarbin, Marco.

In: Investigative Ophthalmology and Visual Science, Vol. 42, No. 12, 17.11.2001, p. 2990-2999.

Research output: Contribution to journalArticle

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T1 - Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane

AU - Wang, H.

AU - Leonard, D. S.

AU - Castellarin, A. A.

AU - Tsukahara, I.

AU - Ninomiya, Y.

AU - Yagi, F.

AU - Cheewatrakoolpong, N.

AU - Sugino, I. K.

AU - Zarbin, Marco

PY - 2001/11/17

Y1 - 2001/11/17

N2 - PURPOSE. To investigate the survival and behavior of retinal pigment epithelium (RPE) microaggregates transplanted onto hydraulically debrided Bruch's membrane and to compare results of using three different vehicles for cell delivery. METHODS. RPE microaggregates obtained from male cats were transplanted onto the tapetal area of female cats after native RPE was debrided. For the control, one of three vehicles was introduced into the debridements. Each transplant or control specimen was analyzed histologically and immunohistochemically. Transplanted male RPE cells were identified by in situ labeling of the cat Y chromosome. RESULTS. Histologically, significant numbers of condensed, darkly stained RPE nuclei were observed in all transplants compared with few TUNEL-positive RPE cells. Cellular retinal-dehyde-binding protein was present up to day 7 in all RPE cells in transplants. In both transplant and control specimens, the antibody against the Ki-67 nuclear antigen labeled some RPE cells at day 3. TUNEL-positive outer nuclear layer nuclei were most frequently observed at day 1, but were much less frequent at 7 days in both transplant and control specimens. CONCLUSIONS. Transplanted RPE appeared to retain at least some markers of differentiation up to 7 days after surgery. Some proliferation of transplanted RPE cells was also seen. Apoptotic cell death of transplanted RPE, as judged by TUNEL staining was observed rarely. RPE transplants imposed no adverse effect on the overlying retina. RPE survival appeared to be similar with each of the three vehicles for cell delivery.

AB - PURPOSE. To investigate the survival and behavior of retinal pigment epithelium (RPE) microaggregates transplanted onto hydraulically debrided Bruch's membrane and to compare results of using three different vehicles for cell delivery. METHODS. RPE microaggregates obtained from male cats were transplanted onto the tapetal area of female cats after native RPE was debrided. For the control, one of three vehicles was introduced into the debridements. Each transplant or control specimen was analyzed histologically and immunohistochemically. Transplanted male RPE cells were identified by in situ labeling of the cat Y chromosome. RESULTS. Histologically, significant numbers of condensed, darkly stained RPE nuclei were observed in all transplants compared with few TUNEL-positive RPE cells. Cellular retinal-dehyde-binding protein was present up to day 7 in all RPE cells in transplants. In both transplant and control specimens, the antibody against the Ki-67 nuclear antigen labeled some RPE cells at day 3. TUNEL-positive outer nuclear layer nuclei were most frequently observed at day 1, but were much less frequent at 7 days in both transplant and control specimens. CONCLUSIONS. Transplanted RPE appeared to retain at least some markers of differentiation up to 7 days after surgery. Some proliferation of transplanted RPE cells was also seen. Apoptotic cell death of transplanted RPE, as judged by TUNEL staining was observed rarely. RPE transplants imposed no adverse effect on the overlying retina. RPE survival appeared to be similar with each of the three vehicles for cell delivery.

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Wang H, Leonard DS, Castellarin AA, Tsukahara I, Ninomiya Y, Yagi F et al. Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane. Investigative Ophthalmology and Visual Science. 2001 Nov 17;42(12):2990-2999.