Site-restricted expression of cytotactin during development of the chicken embryo

K. L. Crossin, S. Hoffman, Martin Grumet, J. P. Thiery, G. M. Edelman

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Abstract

The sequential appearance of the extracellular matrix (ECM) protein, cytotactin, was examined during development of the chicken embryo by immunohistochemical techniques. Although cytotactin was identified as a molecule that mediates glia-neuron interactions, preliminary immunohistochemical localization of the molecule suggested that it was an ECM protein with a widespread but nonetheless more restricted distribution than either fibronectin or laminin. In the present study, it was found that cytotactin is first present in the gastrulating chicken embryo. It appears later in the basement membrane of the developing neural tube and notochord in a temporal sequence beginning in the cephalic regions and proceeding caudally. Between 2 and 3 d of development, the molecule is present at high levels in the early neural crest pathways (surrounding the neural tube and somites) but, in contrast to fibronectin and laminin, is not found in the lateral plate mesoderm or ectoderm. At later times, cytotactin is expressed extensively in the central nervous system, in lesser amounts in the peripheral nervous system, and in a number of non-neural sites, most prominently in all smooth muscles and in basement membranes of lung and kidney. Cytotactin appears in adult tissues with distributions that are similar to those seen in embryonic tissues. The findings raise the possibility that certain ECM proteins contribute to pattern formation in embryogenesis as a result of their restricted expression in a spatiotemporally regulated fashion at some sites but not at others.

Original languageEnglish (US)
Pages (from-to)1917-1930
Number of pages14
JournalJournal of Cell Biology
Volume102
Issue number5
DOIs
StatePublished - Jan 1 1986

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Tenascin
Embryonic Development
Chickens
Extracellular Matrix Proteins
Neural Tube
Laminin
Fibronectins
Basement Membrane
Notochord
Neural Pathways
Somites
Ectoderm
Neural Crest
Peripheral Nervous System
Mesoderm
Tissue Distribution
Neuroglia
Smooth Muscle
Embryonic Structures
Central Nervous System

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Crossin, K. L. ; Hoffman, S. ; Grumet, Martin ; Thiery, J. P. ; Edelman, G. M. / Site-restricted expression of cytotactin during development of the chicken embryo. In: Journal of Cell Biology. 1986 ; Vol. 102, No. 5. pp. 1917-1930.
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Site-restricted expression of cytotactin during development of the chicken embryo. / Crossin, K. L.; Hoffman, S.; Grumet, Martin; Thiery, J. P.; Edelman, G. M.

In: Journal of Cell Biology, Vol. 102, No. 5, 01.01.1986, p. 1917-1930.

Research output: Contribution to journalArticle

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