Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila

K. Nicole Clouse, Scott B. Ferguson, Gertrud Schupbach

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

During Drosophila melanogaster oogenesis, the proper localization of gurken (grk) mRNA and protein is required for the establishment of the dorsal-ventral axis of the egg and future embryo. Squid (Sqd) is an RNA-binding protein that is required for the correct localization and translational regulation of the grk message. We show that Cup and polyA-binding protein (PABP) interact physically with Sqd and with each other in ovaries. We show that cup mutants lay dorsalized eggs, enhance dorsalization of weak sqd alleles, and display defects in grk mRNA localization and Grk protein accumulation. In contrast, pAbp mutants lay ventralized eggs and enhance grk haploinsufficiency. PABP also interacts genetically and biochemically with Encore. These data predict a model in which Cup and Sqd mediate translational repression of unlocalized grk mRNA, and PABP and Enc facilitate translational activation of the message once it is fully localized to the dorsal-anterior region of the oocyte. These data also provide the first evidence of a link between the complex of commonly used trans-acting factors and Enc, a factor that is required for grk translation.

Original languageEnglish (US)
Pages (from-to)713-724
Number of pages12
JournalDevelopmental biology
Volume313
Issue number2
DOIs
StatePublished - Jan 15 2008

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Decapodiformes
Drosophila
Carrier Proteins
Messenger RNA
Eggs
Haploinsufficiency
Oogenesis
Trans-Activators
RNA-Binding Proteins
Drosophila melanogaster
Oocytes
Ovum
Ovary
Proteins
Embryonic Structures
Alleles

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology
  • Developmental Biology

Cite this

Clouse, K. Nicole ; Ferguson, Scott B. ; Schupbach, Gertrud. / Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila. In: Developmental biology. 2008 ; Vol. 313, No. 2. pp. 713-724.
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Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila. / Clouse, K. Nicole; Ferguson, Scott B.; Schupbach, Gertrud.

In: Developmental biology, Vol. 313, No. 2, 15.01.2008, p. 713-724.

Research output: Contribution to journalArticle

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