Stability of luciferase gene expression in a long term period in transgenic eggplant, Solanum melongena

Hiromi Hanyu, Asuka Murata, Enoch Y. Park, Mitsuyasu Okabe, Sharon Billings, Gojko Jelenkovic, Henrik Pedersen, Chee Kok Chin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The luc gene, from firefly, was ligated into the multiple cloning site of the pBI121 plasmid and was then incorporated into Agrobacterium tumefaciens strain LBA4404. This vector containing a CaMV 35S promoter, a NOS terminator, a NPTII gene and a NOS promoter was used to transform 'Hibush' eggplant (Solanum melongena). Genomic DNA from the transformants was analyzed by assay of both PCR and southern blotting, which confirmed the presence of an expected luc fragment. Four transformants were chosen for an extended LUC assay. During the course of one year, LUC activity fluctuated, but was maintained at the initial activity even after 50 weeks. Expression of the luc gene in transgenic calli revealed that fluctuation of the LUC activity was due to changes in the environmental temperature. Luminescence from transgenic eggplant was detected using image analysis and light emission was found to be intense throughout the whole plant.

Original languageEnglish (US)
Pages (from-to)403-407
Number of pages5
JournalPlant Biotechnology
Volume16
Issue number5
DOIs
StatePublished - 1999

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Biotechnology
  • Plant Science

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