Enhancement of reductive dechlorination of native polychlorinated biphenyls (PCBs) was investigated in sediments from two urban sites: Kearny Marsh, in the New Jersey Meadowlands and the Anacostia River, in Washington, DC. Sediments were treated by adding electron acceptors and/or halogenated amendments (25 μM tetrachlorobenzene or pentachloronitrobenzene) and by bioaugmentation with Dehalococcoides ethenogenes strain 195. Treatment effectiveness was assessed by examining changes in congener patterns of the native PCBs and by tracking DNA-based markers of dechlorinating bacteria and reductive dehalogenase genes. The pentachloronitrobenzene treatment stimulated PCB dechlorination in the Anacostia sediment with or without bioaugmentation. The tetrachlorobenzene treatment stimulated dechlorination in the Kearny Marsh sediment, but to a lesser extent. Dechlorination potential was further tested by spiking subcultures from the various treatments with PCB116 and comparing dechlorination rates in previously biostimulated microcosms versus those in controls. Chloroflexi including Dehalococcoides were detected in both site sediments using polymerase chain reaction (PCR) analysis.

Original languageAmerican English
Pages (from-to)215-219
Number of pages5
JournalACS, Division of Environmental Chemistry - Preprints of Extended Abstracts
Issue number2
StatePublished - 2008
EventAmerican Chemical Society Division of Environmental Chemistry Preprints of Extended Abstracts 2008 - Philadelphia, United States
Duration: Aug 17 2008Aug 21 2008

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Environmental Chemistry


Dive into the research topics of 'Stimulation of reductive dechlorination of PCBs in contaminated sediments'. Together they form a unique fingerprint.

Cite this