Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies

Xiong Gao, Jiayi Qi, Chi Tang Ho, Bin Li, Jingjing Mu, Yuting Zhang, Huiping Hu, Weipeng Mo, Zhongzheng Chen, Yizhen Xie

Research output: Contribution to journalArticle

Abstract

Ganoderma leucocontextum is a new species of Ganoderma discovered in 2014. Up to now, the structural characteristics and immunoregulatory activity of its polysaccharides remain virtually unknown. In this study, a water-soluble polysaccharide termed, GLP-3, was purified from G. leucocontextum by ultrafiltration and column chromatography. The results revealed that GLP-3 mainly consisted of glucose (92.7 %) and its weight average molecular weight was 159.7 kDa. The structural analysis indicated that the backbone of GLP-3 was →4)-α-D-Glcp-(1→4,6)-β-D-Glcp-(1→ with a β-Glcp-(1→ branch. Atomic force microscopy and Congo red experiments revealed that GLP-3 might possess a globular structure with triple-helix conformation in water. Moreover, GLP-3 was recognized by toll-like receptor 2 (TLR2) and exerted immunomodulatory effects via activating mitogen-activated protein kinases (MAPKs), phosphatidylinositol-3-kinase (PI3K)/Akt and nuclear factor-κB (NF-κB) signaling pathways in RAW 264.7 macrophages. Collectively, these results suggested that GLP-3 could be developed as a potential functional food ingredient for immunomodulation.

Original languageEnglish (US)
Article number116874
JournalCarbohydrate Polymers
Volume249
DOIs
StatePublished - Dec 1 2020

All Science Journal Classification (ASJC) codes

  • Materials Chemistry
  • Polymers and Plastics
  • Organic Chemistry

Keywords

  • Ganoderma leucocontextum
  • Immunostimulatory activity
  • Polysaccharide
  • Structure
  • Toll-like receptor 2

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