Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry

TY - JOUR

T1 - Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry

AU - Shao, Xi

AU - Chen, Xiaoxin

AU - Badmaev, Vladimir

AU - Ho, Chi Tang

AU - Sang, Shengmin

PY - 2010/6

Y1 - 2010/6

N2 - Pterostilbene, the dimethoxy derivative of resveratrol, has drawn much attention recently due to its potential beneficial health effects. The metabolic fate of pterostilbene, however, is not well understood. In the present study, we identified nine novel mouse urinary pterostilbene metabolites, pterostilbene glucuronide, pterostilbene sulfate, mono-demethylated pterostilbene glucuronide, mono-demethylated pterostilbene sulfate, mono-hydroxylated pterostilbene, mono-hydroxylated pterostilbene glucuronide, mono-hydroxylated pterostilbene sulfate, and mono-hydroxylated pterostilbene glucuronide sulfate, using liquid chromatography/atmospheric pressure chemical ionization and electrospray ionization tandem mass spectrometry. The structures of these metabolites were confirmed by analyzing the MSn (n=1-3) spectra. To our knowledge, this is the first report of the identification of urinary metabolites of pterostilbene in mice.

AB - Pterostilbene, the dimethoxy derivative of resveratrol, has drawn much attention recently due to its potential beneficial health effects. The metabolic fate of pterostilbene, however, is not well understood. In the present study, we identified nine novel mouse urinary pterostilbene metabolites, pterostilbene glucuronide, pterostilbene sulfate, mono-demethylated pterostilbene glucuronide, mono-demethylated pterostilbene sulfate, mono-hydroxylated pterostilbene, mono-hydroxylated pterostilbene glucuronide, mono-hydroxylated pterostilbene sulfate, and mono-hydroxylated pterostilbene glucuronide sulfate, using liquid chromatography/atmospheric pressure chemical ionization and electrospray ionization tandem mass spectrometry. The structures of these metabolites were confirmed by analyzing the MSn (n=1-3) spectra. To our knowledge, this is the first report of the identification of urinary metabolites of pterostilbene in mice.

UR - http://www.scopus.com/inward/record.url?scp=77953213678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953213678&partnerID=8YFLogxK

U2 - https://doi.org/10.1002/rcm.4579

DO - https://doi.org/10.1002/rcm.4579

M3 - Article

C2 - 20499322

VL - 24

SP - 1770

EP - 1778

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 12

ER -