Structure of the mouse Stat 3/5 locus: Evolution from Drosophila to zebrafish to mouse

Keiko Miyoshi; Yongzhi Cui; Greg Riedlinger; Phyllis Robinson; Jessica Lehoczky; Leonard Zon; Takami Oka; Ken Dewar; Lothar Hennighausen

doi:10.1006/geno.2000.6433

Structure of the mouse Stat 3/5 locus: Evolution from Drosophila to zebrafish to mouse

Keiko Miyoshi
, Yongzhi Cui
, Greg Riedlinger
, Phyllis Robinson
, Jessica Lehoczky
, Leonard Zon
, Takami Oka
, Ken Dewar
, Lothar Hennighausen

Research output: Contribution to journal › Article › peer-review

Abstract

Signal transducers and activators of transcription (Stat) are transcription factors that can be activated by many cytokines. While Drosophila contains only one Stat (d-Stat), mammals contain seven, with STATs 3, 5a, and 5b being the closest functional relatives. To understand the evolutionary relationship between d-Stat and vertebrate STATs 3 and 5, we isolated, sequenced, and analyzed the zebrafish Stat3 (z-Stat3) gene and a 500-kb region spanning mouse chromosome 11, 60.5 cM containing three Stat genes (m-Stats). Within this region we identified the genes encoding m-Stats 3, 5a, and 5b, Cnp1, Hcrt/Orexin, Ptrf, GCN5, mDj11, and four new genes. The 5′ ends of the m-Stat5a and m-Stat5b genes are juxtaposed to each other, and the 3′ ends of the m-Stat3 and Stat5a genes face each other. While the m-Stat5a and m-Stat3 genes have one promoter each, which are active in many tissues, the m-Stat5b gene acquired two distinct promoters. The distal promoter is expressed ubiquitously, and transcription from the proximal promoter is restricted to liver, muscle, and mammary tissue. Through a comparison of exon-intron boundaries from the m-Stat3, m-Stat5a, and m-Stat5b, z-Stat3, and d-Stat genes, we deduced their evolutionary relationship. We propose that the Stat3 and Stat5 lineages are derived from the duplication of a common primordial gene and that d-Stat is a part of the Stat5 lineage.

Original language	American English
Pages (from-to)	150-155
Number of pages	6
Journal	Genomics
Volume	71
Issue number	2
DOIs	https://doi.org/10.1006/geno.2000.6433
State	Published - Jan 15 2001
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1006/geno.2000.6433

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title = "Structure of the mouse Stat 3/5 locus: Evolution from Drosophila to zebrafish to mouse",

abstract = "Signal transducers and activators of transcription (Stat) are transcription factors that can be activated by many cytokines. While Drosophila contains only one Stat (d-Stat), mammals contain seven, with STATs 3, 5a, and 5b being the closest functional relatives. To understand the evolutionary relationship between d-Stat and vertebrate STATs 3 and 5, we isolated, sequenced, and analyzed the zebrafish Stat3 (z-Stat3) gene and a 500-kb region spanning mouse chromosome 11, 60.5 cM containing three Stat genes (m-Stats). Within this region we identified the genes encoding m-Stats 3, 5a, and 5b, Cnp1, Hcrt/Orexin, Ptrf, GCN5, mDj11, and four new genes. The 5′ ends of the m-Stat5a and m-Stat5b genes are juxtaposed to each other, and the 3′ ends of the m-Stat3 and Stat5a genes face each other. While the m-Stat5a and m-Stat3 genes have one promoter each, which are active in many tissues, the m-Stat5b gene acquired two distinct promoters. The distal promoter is expressed ubiquitously, and transcription from the proximal promoter is restricted to liver, muscle, and mammary tissue. Through a comparison of exon-intron boundaries from the m-Stat3, m-Stat5a, and m-Stat5b, z-Stat3, and d-Stat genes, we deduced their evolutionary relationship. We propose that the Stat3 and Stat5 lineages are derived from the duplication of a common primordial gene and that d-Stat is a part of the Stat5 lineage.",

author = "Keiko Miyoshi and Yongzhi Cui and Greg Riedlinger and Phyllis Robinson and Jessica Lehoczky and Leonard Zon and Takami Oka and Ken Dewar and Lothar Hennighausen",

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AU - Miyoshi, Keiko

AU - Cui, Yongzhi

AU - Riedlinger, Greg

AU - Robinson, Phyllis

AU - Lehoczky, Jessica

AU - Zon, Leonard

AU - Oka, Takami

AU - Dewar, Ken

AU - Hennighausen, Lothar

PY - 2001/1/15

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N2 - Signal transducers and activators of transcription (Stat) are transcription factors that can be activated by many cytokines. While Drosophila contains only one Stat (d-Stat), mammals contain seven, with STATs 3, 5a, and 5b being the closest functional relatives. To understand the evolutionary relationship between d-Stat and vertebrate STATs 3 and 5, we isolated, sequenced, and analyzed the zebrafish Stat3 (z-Stat3) gene and a 500-kb region spanning mouse chromosome 11, 60.5 cM containing three Stat genes (m-Stats). Within this region we identified the genes encoding m-Stats 3, 5a, and 5b, Cnp1, Hcrt/Orexin, Ptrf, GCN5, mDj11, and four new genes. The 5′ ends of the m-Stat5a and m-Stat5b genes are juxtaposed to each other, and the 3′ ends of the m-Stat3 and Stat5a genes face each other. While the m-Stat5a and m-Stat3 genes have one promoter each, which are active in many tissues, the m-Stat5b gene acquired two distinct promoters. The distal promoter is expressed ubiquitously, and transcription from the proximal promoter is restricted to liver, muscle, and mammary tissue. Through a comparison of exon-intron boundaries from the m-Stat3, m-Stat5a, and m-Stat5b, z-Stat3, and d-Stat genes, we deduced their evolutionary relationship. We propose that the Stat3 and Stat5 lineages are derived from the duplication of a common primordial gene and that d-Stat is a part of the Stat5 lineage.

AB - Signal transducers and activators of transcription (Stat) are transcription factors that can be activated by many cytokines. While Drosophila contains only one Stat (d-Stat), mammals contain seven, with STATs 3, 5a, and 5b being the closest functional relatives. To understand the evolutionary relationship between d-Stat and vertebrate STATs 3 and 5, we isolated, sequenced, and analyzed the zebrafish Stat3 (z-Stat3) gene and a 500-kb region spanning mouse chromosome 11, 60.5 cM containing three Stat genes (m-Stats). Within this region we identified the genes encoding m-Stats 3, 5a, and 5b, Cnp1, Hcrt/Orexin, Ptrf, GCN5, mDj11, and four new genes. The 5′ ends of the m-Stat5a and m-Stat5b genes are juxtaposed to each other, and the 3′ ends of the m-Stat3 and Stat5a genes face each other. While the m-Stat5a and m-Stat3 genes have one promoter each, which are active in many tissues, the m-Stat5b gene acquired two distinct promoters. The distal promoter is expressed ubiquitously, and transcription from the proximal promoter is restricted to liver, muscle, and mammary tissue. Through a comparison of exon-intron boundaries from the m-Stat3, m-Stat5a, and m-Stat5b, z-Stat3, and d-Stat genes, we deduced their evolutionary relationship. We propose that the Stat3 and Stat5 lineages are derived from the duplication of a common primordial gene and that d-Stat is a part of the Stat5 lineage.

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ER -

Structure of the mouse Stat 3/5 locus: Evolution from Drosophila to zebrafish to mouse

Abstract

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