TY - JOUR
T1 - Structures and physicochemical characterization of enzyme extracted oil bodies from rice bran
AU - Xu, Duoxia
AU - Gao, Qianru
AU - Ma, Ningning
AU - Hao, Jia
AU - Yuan, Yingmao
AU - Zhang, Min
AU - Cao, Yanping
AU - Ho, Chi Tang
N1 - Funding Information: This research was funded by 13th Five-Year the State Key Development Program ( 2018YFD0401101 ), the National Natural Science Foundation of China ( 32072216 , 31771976 , 31871808 ), School Level Cultivation Fund of Beijing Technology and Business University for Distinguished and Excellent Young Scholars ( BTBUYP2020 ), Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan ( CIT & TCD201804018 ), Construction of Service Capability of Scientific and Technological Innovation ( PXM2019_014213_000010 , PXM2018_014213_000033 , PXM2018_014213_000014 , PXM2018_014213_000041 , 19005902054 , 19005857058 ), China Scholarship Council , Cultivation and Development of Innovation Base ( Z171100002217019 ). The authors are grateful to Tsinghua University Branch of China National Center Protein Sciences (Beijing, China) for providing the facility support of Confocal laser microscopy and Cryo-SEM. Funding Information: This research was funded by 13th Five-Year the State Key Development Program (2018YFD0401101), the National Natural Science Foundation of China (32072216, 31771976, 31871808), School Level Cultivation Fund of Beijing Technology and Business University for Distinguished and Excellent Young Scholars (BTBUYP2020), Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan (CIT & TCD201804018), Construction of Service Capability of Scientific and Technological Innovation (PXM2019_014213_000010, PXM2018_014213_000033, PXM2018_014213_000014, PXM2018_014213_000041, 19005902054, 19005857058), China Scholarship Council, Cultivation and Development of Innovation Base (Z171100002217019). The authors are grateful to Tsinghua University Branch of China National Center Protein Sciences (Beijing, China) for providing the facility support of Confocal laser microscopy and Cryo-SEM. Publisher Copyright: © 2020
PY - 2021/1
Y1 - 2021/1
N2 - Oil bodies (OBs) are a natural delivery system rich in functional ingredients. This study analyzed the structures, physical properties and chemical compositions of rice bran oil bodies (RBOBs) extracted by three different enzymes (Plant exacted enzyme-RBOBP, Xylanase-RBOBX, and their mixture-RBOBM), respectively. The particle size distribution and zeta-potential of RBOBs were determined. The structure of RBOBs was observed by using Cryo-scanning electron microscopy (Cryo-SEM) and confocal laser scanning microscopy (CLSM). RBOBs were also characterized by acrylamide electrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for protein identification. The fatty acid compositions of RBOBs were analyzed by UPLC-Q-Exactive Orbitrap/MS. The three RBOBs exhibited polydispersed size distributions. The RBOBM showed the most uniform distribution due to the highest protein content and strongest steric hindrance. X-ray diffraction (XRD) patterns showed that RBOBs were amorphous structure. SDS-PAGE proved that RBOB surface contained OB related proteins and a large number of extraneous proteins. In addition to the previously known oleosin, some new proteins (germin-like protein 16, osmotin, Histone H4, putative, expressed protein, calmodulin, putative glycopeptide et al.) were identified. The fatty acid composition of RBOB was similar to that of rice bran oil. It was useful to study the natural plant OB delivery system.
AB - Oil bodies (OBs) are a natural delivery system rich in functional ingredients. This study analyzed the structures, physical properties and chemical compositions of rice bran oil bodies (RBOBs) extracted by three different enzymes (Plant exacted enzyme-RBOBP, Xylanase-RBOBX, and their mixture-RBOBM), respectively. The particle size distribution and zeta-potential of RBOBs were determined. The structure of RBOBs was observed by using Cryo-scanning electron microscopy (Cryo-SEM) and confocal laser scanning microscopy (CLSM). RBOBs were also characterized by acrylamide electrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for protein identification. The fatty acid compositions of RBOBs were analyzed by UPLC-Q-Exactive Orbitrap/MS. The three RBOBs exhibited polydispersed size distributions. The RBOBM showed the most uniform distribution due to the highest protein content and strongest steric hindrance. X-ray diffraction (XRD) patterns showed that RBOBs were amorphous structure. SDS-PAGE proved that RBOB surface contained OB related proteins and a large number of extraneous proteins. In addition to the previously known oleosin, some new proteins (germin-like protein 16, osmotin, Histone H4, putative, expressed protein, calmodulin, putative glycopeptide et al.) were identified. The fatty acid composition of RBOB was similar to that of rice bran oil. It was useful to study the natural plant OB delivery system.
KW - Microstructure
KW - Oil body
KW - Physicochemical characterization
KW - Rice bran
KW - Stability
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U2 - https://doi.org/10.1016/j.lwt.2020.109982
DO - https://doi.org/10.1016/j.lwt.2020.109982
M3 - Article
SN - 0023-6438
VL - 135
JO - LWT - Food Science and Technology
JF - LWT - Food Science and Technology
M1 - 109982
ER -