Synthesis of capped RNA using a DMT group as a purification handle

Elizabeth Veliath, Barbara L. Gaffney, Roger A. Jones

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


We report a new method for synthesis of capped RNA or 2′-OMe RNA that uses a N2-4,4′-dimethoxytrityl (DMT) group as a lipophilic purification handle to allow convenient isolation and purification of the capped RNA. The DMT group is easily removed under mild conditions without degradation of the cap. We have used this approach to prepare capped 10- and 20-mers. This method is compatible with the many condensation reactions that have been reported for preparation of capped RNA or cap analogues.

Original languageAmerican English
Pages (from-to)40-52
Number of pages13
JournalNucleosides, Nucleotides and Nucleic Acids
Issue number1
StatePublished - Jan 2 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics


  • Modified oligonucleotides
  • RNA oligonucleotides
  • Synthetic methodology


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