Synthesis of capped RNA using a DMT group as a purification handle

TY - JOUR

T1 - Synthesis of capped RNA using a DMT group as a purification handle

AU - Veliath, Elizabeth

AU - Gaffney, Barbara L.

AU - Jones, Roger A.

N1 - Funding Information: This work was supported by NIH grant GM079760.

PY - 2014/1/2

Y1 - 2014/1/2

N2 - We report a new method for synthesis of capped RNA or 2′-OMe RNA that uses a N2-4,4′-dimethoxytrityl (DMT) group as a lipophilic purification handle to allow convenient isolation and purification of the capped RNA. The DMT group is easily removed under mild conditions without degradation of the cap. We have used this approach to prepare capped 10- and 20-mers. This method is compatible with the many condensation reactions that have been reported for preparation of capped RNA or cap analogues.

AB - We report a new method for synthesis of capped RNA or 2′-OMe RNA that uses a N2-4,4′-dimethoxytrityl (DMT) group as a lipophilic purification handle to allow convenient isolation and purification of the capped RNA. The DMT group is easily removed under mild conditions without degradation of the cap. We have used this approach to prepare capped 10- and 20-mers. This method is compatible with the many condensation reactions that have been reported for preparation of capped RNA or cap analogues.

KW - Modified oligonucleotides

KW - RNA oligonucleotides

KW - Synthetic methodology

UR - http://www.scopus.com/inward/record.url?scp=84897691274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897691274&partnerID=8YFLogxK

U2 - https://doi.org/10.1080/15257770.2013.864417

DO - https://doi.org/10.1080/15257770.2013.864417

M3 - Article

C2 - 24588755

SN - 0732-8311

VL - 33

SP - 40

EP - 52

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

IS - 1

ER -