Synthesis of capped RNA using a DMT group as a purification handle

Elizabeth Veliath, Barbara L. Gaffney, Roger Jones

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We report a new method for synthesis of capped RNA or 2′-OMe RNA that uses a N2-4,4′-dimethoxytrityl (DMT) group as a lipophilic purification handle to allow convenient isolation and purification of the capped RNA. The DMT group is easily removed under mild conditions without degradation of the cap. We have used this approach to prepare capped 10- and 20-mers. This method is compatible with the many condensation reactions that have been reported for preparation of capped RNA or cap analogues.

Original languageEnglish (US)
Pages (from-to)40-52
Number of pages13
JournalNucleosides, Nucleotides and Nucleic Acids
Volume33
Issue number1
DOIs
StatePublished - Jan 2 2014

Fingerprint

Purification
RNA Cap Analogs
RNA
Condensation reactions
Degradation

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Medicine
  • Biochemistry

Keywords

  • Modified oligonucleotides
  • RNA oligonucleotides
  • Synthetic methodology

Cite this

Veliath, Elizabeth ; Gaffney, Barbara L. ; Jones, Roger. / Synthesis of capped RNA using a DMT group as a purification handle. In: Nucleosides, Nucleotides and Nucleic Acids. 2014 ; Vol. 33, No. 1. pp. 40-52.
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Synthesis of capped RNA using a DMT group as a purification handle. / Veliath, Elizabeth; Gaffney, Barbara L.; Jones, Roger.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 33, No. 1, 02.01.2014, p. 40-52.

Research output: Contribution to journalArticle

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