TbMP44 is essential for RNA editing and structural integrity of the editosome in Trypanosoma brucei

Bingbing Wang, Nancy Lewis Ernst, Setareh S. Palazzo, Aswini K. Panigrahi, Reza Salavati, Kenneth Stuart

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

RNA editing produces mature mitochondrial mRNAs in trypanosomatids by the insertion and deletion of uridylates. It is catalyzed by a multiprotein complex, the editosome. We identified ThMP44 among the components of enriched editosomes by a combination of mass spectrometry and DNA sequence database analysis. Inactivation of an ectopic TbMP44 allele in cells in which the endogenous alleles were disrupted abolished RNA editing, inhibited cell growth, and was eventually lethal to bloodstream form trypanosomes. Loss of TbMP44 mRNA was followed initially by a reduction in the editosome sedimentation coefficient and then by the absence of other editosome proteins despite the presence of the mRNA. Reactivation of TbMP44 gene expression resulted in the resumption of cell growth and the reappearance of editosomes. These data indicate that TbMP44 is a component of the editosome that is essential for editing and critical for the structural integrity of the editosome.

Original languageEnglish (US)
Pages (from-to)578-587
Number of pages10
JournalEukaryotic Cell
Volume2
Issue number3
DOIs
StatePublished - Jun 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Microbiology

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