Telomere length measurement by a novel Luminex-based assay

A blinded comparison to Southern blot

Brandon L. Pierce, Farzana Jasmine, Shantanu Roy, Chenan Zhang, Abraham Aviv, Steven C. Hunt, Habibul Ahsan, Muhammad G. Kibriya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Telomere length (TL) is a potential biomarker of aging and age-related disease risk. We recently published a novel Luminex-based method for high-throughput, low-cost TL measurement. Here we describe a blinded comparison of the Luminex method to Southern blot, the most precise TL measurement method. Luminex and Southern blot measurements for the same 50 DNA samples were taken in two independent laboratories; each sample was measured twice, several months apart. The inter-assay CV for Luminex ranged from 5.5 to 9.1 (depending on CV estimation method), and Southern blot CV from 1.0 to 1.7. Both measures were inversely associated with age. The correlation between the repeated measurements was 0.66 for Luminex and 0.97 for Southern blot. The correlation between Southern blot and Luminex was 0.65 in round 1 and 0.75 in round 2, and the relationship showed no evidence of non-linearity. Our results demonstrate that the Luminex assay is a valid and reproducible method for high-throughput TL measurement. The Luminex assay involves no DNA amplification, which may make Luminex an attractive alternative to PCR-based TL measurement.

Original languageEnglish (US)
Pages (from-to)18-23
Number of pages6
JournalInternational Journal of Molecular Epidemiology and Genetics
Volume7
Issue number1
StatePublished - Jan 1 2016

Fingerprint

Telomere
Southern Blotting
DNA
Biomarkers
Costs and Cost Analysis
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)
  • Genetics
  • Epidemiology

Keywords

  • Aging
  • Luminex
  • Southern blot
  • Telomere length
  • Validation
  • qPCR

Cite this

Pierce, Brandon L. ; Jasmine, Farzana ; Roy, Shantanu ; Zhang, Chenan ; Aviv, Abraham ; Hunt, Steven C. ; Ahsan, Habibul ; Kibriya, Muhammad G. / Telomere length measurement by a novel Luminex-based assay : A blinded comparison to Southern blot. In: International Journal of Molecular Epidemiology and Genetics. 2016 ; Vol. 7, No. 1. pp. 18-23.
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Pierce, BL, Jasmine, F, Roy, S, Zhang, C, Aviv, A, Hunt, SC, Ahsan, H & Kibriya, MG 2016, 'Telomere length measurement by a novel Luminex-based assay: A blinded comparison to Southern blot', International Journal of Molecular Epidemiology and Genetics, vol. 7, no. 1, pp. 18-23.

Telomere length measurement by a novel Luminex-based assay : A blinded comparison to Southern blot. / Pierce, Brandon L.; Jasmine, Farzana; Roy, Shantanu; Zhang, Chenan; Aviv, Abraham; Hunt, Steven C.; Ahsan, Habibul; Kibriya, Muhammad G.

In: International Journal of Molecular Epidemiology and Genetics, Vol. 7, No. 1, 01.01.2016, p. 18-23.

Research output: Contribution to journalArticle

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