@inbook{b04feb6f7ed1403e85be2be4ac6682be,
title = "The continuing development of E. Coli as a heterologous host for complex natural product biosynthesis",
abstract = "Heterologous biosynthesis of natural products is meant to enable access to the vast array of valuable properties associated with these compounds. Often motivated by limitations inherent in native production hosts, the heterologous biosynthetic process begins with a candidate host regarded as technically advanced relative to original producing organisms. Given this requirement, E. coli has been a top choice for heterologous biosynthesis attempts as associated recombinant tools emerged and continue to develop. However, success requires overcoming challenges associated with natural product formation, including complex biosynthetic pathways and the need for metabolic support. These two challenges have been heavily featured in cellular engineering efforts completed to position E. coli as a viable surrogate host. This chapter outlines steps taken to engineer E. coli with an emphasis on genetic manipulations designed to support the heterologous production of polyketide, nonribosomal peptide, and similarly complex natural products.",
keywords = "Cellular design, E. coli, Heterologous biosynthesis, Natural products, Nonribosomal peptide, Polyketide",
author = "Haoran Zhang and Lei Fang and Osburne, {Marcia S.} and Pfeifer, {Blaine A.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = feb,
day = "1",
doi = "10.1007/978-1-4939-3375-4_8",
language = "American English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "121--134",
booktitle = "Methods in Molecular Biology",
}