The cortical actin-membrane cytoskeleton of unfertilized sea urchin eggs: Analysis of the spatial organization and relationship of filamentous actin, nonfilamentous actin, and egg spectrin

Edward Bonder, Douglas J. Fishkind, Nina M. Cotran, David A. Begg

Research output: Contribution to journalArticle

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Abstract

Whole mounts, cryosections, and isolated cortices of unfertilized sea urchin eggs were probed with fluorescent phalloidin, anti-actin and anti-egg spectrin antibodies to investigate the organizational state of the cortically associated actin-membrane cytoskeleton. Filamentous actin and egg spectrin were localized to the plasma membrane, within microvillar and nonmicrovillar domains. The nonmicrovillar filamentous actin was located immediately subjacent to the microvilli forming an extensive interconnecting network along the inner surface of the plasma membrane. The organization of this filamentous actin network precisely correlated with the positioning of the underlying cortical granules. The cortical cytoplasm did not contain any detectable filamentous actin, but instead contained a sequestered domain of nonfilamentous actin. Spectrin was localized to the cytoplasmic surface of the plasma membrane with concentrated foci co-localized with the filamentous actin present in microvilli. Spectrin was also observed to coat the surfaces of cortical granules as well as other populations of intracellular vesicles. On the basis of light microscopic morphology, intracellular distribution, and co-isolation with the egg cortex, some of these spectrin-coated organelles represent acidic vesicles. Identification of an elaborate organization of inter-related domains of actin (filamentous and nonfilamentous) and spectrin forming the cortical membrane cytoskeleton provides insight into the fundamental mechanisms for early membrane restructuring during embryogenesis. Additionally, the localization of spectrin to the surface of intracellular vesicles is indicative of its newly identified functional roles in membrane trafficking, membrane biogenesis and cellular differentiation.

Original languageEnglish (US)
Pages (from-to)327-341
Number of pages15
JournalDevelopmental biology
Volume134
Issue number2
DOIs
StatePublished - Jan 1 1989

Fingerprint

Spectrin
Spatial Analysis
Sea Urchins
Actin Cytoskeleton
Eggs
Ovum
Actins
Membranes
Cell Membrane
Microvilli
Phalloidine
Cytoskeleton
Organelles
Embryonic Development
Cytoplasm
Light
Antibodies

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

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title = "The cortical actin-membrane cytoskeleton of unfertilized sea urchin eggs: Analysis of the spatial organization and relationship of filamentous actin, nonfilamentous actin, and egg spectrin",
abstract = "Whole mounts, cryosections, and isolated cortices of unfertilized sea urchin eggs were probed with fluorescent phalloidin, anti-actin and anti-egg spectrin antibodies to investigate the organizational state of the cortically associated actin-membrane cytoskeleton. Filamentous actin and egg spectrin were localized to the plasma membrane, within microvillar and nonmicrovillar domains. The nonmicrovillar filamentous actin was located immediately subjacent to the microvilli forming an extensive interconnecting network along the inner surface of the plasma membrane. The organization of this filamentous actin network precisely correlated with the positioning of the underlying cortical granules. The cortical cytoplasm did not contain any detectable filamentous actin, but instead contained a sequestered domain of nonfilamentous actin. Spectrin was localized to the cytoplasmic surface of the plasma membrane with concentrated foci co-localized with the filamentous actin present in microvilli. Spectrin was also observed to coat the surfaces of cortical granules as well as other populations of intracellular vesicles. On the basis of light microscopic morphology, intracellular distribution, and co-isolation with the egg cortex, some of these spectrin-coated organelles represent acidic vesicles. Identification of an elaborate organization of inter-related domains of actin (filamentous and nonfilamentous) and spectrin forming the cortical membrane cytoskeleton provides insight into the fundamental mechanisms for early membrane restructuring during embryogenesis. Additionally, the localization of spectrin to the surface of intracellular vesicles is indicative of its newly identified functional roles in membrane trafficking, membrane biogenesis and cellular differentiation.",
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The cortical actin-membrane cytoskeleton of unfertilized sea urchin eggs : Analysis of the spatial organization and relationship of filamentous actin, nonfilamentous actin, and egg spectrin. / Bonder, Edward; Fishkind, Douglas J.; Cotran, Nina M.; Begg, David A.

In: Developmental biology, Vol. 134, No. 2, 01.01.1989, p. 327-341.

Research output: Contribution to journalArticle

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T1 - The cortical actin-membrane cytoskeleton of unfertilized sea urchin eggs

T2 - Analysis of the spatial organization and relationship of filamentous actin, nonfilamentous actin, and egg spectrin

AU - Bonder, Edward

AU - Fishkind, Douglas J.

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AU - Begg, David A.

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