The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation

Vanessa L. Horner, Andreas Czank, Janet K. Jang, Navjot Singh, Byron C. Williams, Jaakko Puro, Eric Kubli, Steven D. Hanes, Kim McKim, Mariana F. Wolfner, Michael L. Goldberg

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Activation of mature oocytes initiates development by releasing the prior arrest of female meiosis, degrading certain maternal mRNAs while initiating the translation of others, and modifying egg coverings. In vertebrates and marine invertebrates, the fertilizing sperm triggers activation events through a rise in free calcium within the egg. In insects, egg activation occurs independently of sperm and is instead triggered by passage of the egg through the female reproductive tract [1]; it is unknown whether calcium signaling is involved. We report here that mutations in sarah, which encodes an inhibitor of the calcium-dependent phosphatase calcineurin, disrupt several aspects of egg activation in Drosophila. Eggs laid by sarah mutant females arrest in anaphase of meiosis I and fail to fully polyadenylate and translate bicoid mRNA. Furthermore, sarah mutant eggs show elevated cyclin B levels, indicating a failure to inactivate M-phase promoting factor (MPF). Taken together, these results demonstrate that calcium signaling is involved in Drosophila egg activation and suggest a molecular mechanism for the sarah phenotype. We also find the conversion of the sperm nucleus into a functional male pronucleus is compromised in sarah mutant eggs, indicating that the Drosophila egg's competence to support male pronuclear maturation is acquired during activation.

Original languageEnglish (US)
Pages (from-to)1441-1446
Number of pages6
JournalCurrent Biology
Volume16
Issue number14
DOIs
StatePublished - Jul 25 2006

Fingerprint

Drosophila
Ovum
Chemical activation
meiosis
mutants
spermatozoa
insect eggs
Eggs
Calcium
Spermatozoa
calcium
sperm capacitation
pronucleus
Calcium Signaling
anaphase
Meiosis
cyclins
mitosis
translation (genetics)
oocytes

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • DEVBIO

Cite this

Horner, V. L., Czank, A., Jang, J. K., Singh, N., Williams, B. C., Puro, J., ... Goldberg, M. L. (2006). The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation. Current Biology, 16(14), 1441-1446. https://doi.org/10.1016/j.cub.2006.06.024
Horner, Vanessa L. ; Czank, Andreas ; Jang, Janet K. ; Singh, Navjot ; Williams, Byron C. ; Puro, Jaakko ; Kubli, Eric ; Hanes, Steven D. ; McKim, Kim ; Wolfner, Mariana F. ; Goldberg, Michael L. / The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation. In: Current Biology. 2006 ; Vol. 16, No. 14. pp. 1441-1446.
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Horner, VL, Czank, A, Jang, JK, Singh, N, Williams, BC, Puro, J, Kubli, E, Hanes, SD, McKim, K, Wolfner, MF & Goldberg, ML 2006, 'The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation', Current Biology, vol. 16, no. 14, pp. 1441-1446. https://doi.org/10.1016/j.cub.2006.06.024

The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation. / Horner, Vanessa L.; Czank, Andreas; Jang, Janet K.; Singh, Navjot; Williams, Byron C.; Puro, Jaakko; Kubli, Eric; Hanes, Steven D.; McKim, Kim; Wolfner, Mariana F.; Goldberg, Michael L.

In: Current Biology, Vol. 16, No. 14, 25.07.2006, p. 1441-1446.

Research output: Contribution to journalArticle

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T1 - The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation

AU - Horner, Vanessa L.

AU - Czank, Andreas

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AU - Singh, Navjot

AU - Williams, Byron C.

AU - Puro, Jaakko

AU - Kubli, Eric

AU - Hanes, Steven D.

AU - McKim, Kim

AU - Wolfner, Mariana F.

AU - Goldberg, Michael L.

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Horner VL, Czank A, Jang JK, Singh N, Williams BC, Puro J et al. The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation. Current Biology. 2006 Jul 25;16(14):1441-1446. https://doi.org/10.1016/j.cub.2006.06.024