The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition

Joel V. Tamayo, Takamasa Teramoto, Seema Chatterjee, Traci M.Tanaka Hall, Elizabeth R. Gavis

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3′ untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.

Original languageEnglish (US)
Pages (from-to)150-161
Number of pages12
JournalCell Reports
Volume19
Issue number1
DOIs
StatePublished - Apr 4 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Drosophila
  • Glorund
  • RNA-binding protein
  • development
  • hnRNP
  • hnRNP F
  • hnRNP H
  • nanos
  • post-transcriptional regulation
  • translational control
  • translational repressor

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