The effect of total lymphoid irradiation and low-dose steroids on T lymphocyte populations in multiple sclerosis: Correlation with clinical and MRI status

Christine Rohowsky-Kochan, Donna Molinaro, Corinne Devereux, Raymond Troiano, Shalini Bansil, George Zito, Leo Wolansky, Annette Jotkowitz, Thomas Denny, James Oleske, Peter Dowling, Stuart D. Cook

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We have monitored the cell surface phanotypic changes occurring in T, B and NK cells of chronic progressive multiple sclerosis (MS) patients after total lymphoid irradiation (TLI) plus low-dose prednisone (TLI-LDP) therapy in comparison to sham TLI-LDP. TLI-LDP resulted in a marked reduction in the relative and absolute number of total CD3+ T cells, CD4+ helper T cells, CD4+CD45RA+ naive T cells and CD19+ B cells for at least 1 year after treatment. No change occurred in the percent CD8+ T cells although the number of these cells declined after radiotherapy. The CD4/CD8 T cell ratio was also decreased. The relative percent of CD16+ NK cells increased steadily after TLI-LDP while the number of NK cells transiently declined but returned to baseline values 1 year later. An increase in the percent of CD2+CD3- cells and a decrease in their number after therapy was also observed. In contrast, no significant changes in the number of T, B or NK cells were seen in the MS patients receiving sham TLI-LDP. These results provide further evidence that radiotherapy causes a reduction of immunocompetent T and B cells and that a population of possibly NK cells and/or immature T cells appears to be repopulating the circulation after TLI. In addition, a correlation was observed between alterations in lymphocyte populations and the presence or absence of contrast enhancing or new T2 lesions on brain magnetic resonance imaging (MRI) in the TLI-LDP treated MS patients. Patients devoid of contrast enhancing or new T2 lesions had a decreased percentage of CD3+ and CD4+ T cells prior to therapy and at six months following TLI-LDP compared to patients with such lesions. An association was also observed between stability in disease activity as determined on the Expanded Disability Status Scale and relative values of CD3+ T cells.

Original languageEnglish (US)
Pages (from-to)182-192
Number of pages11
JournalJournal of the Neurological Sciences
Volume152
Issue number2
DOIs
StatePublished - Nov 25 1997

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Neurology

Keywords

  • Lymphocytes
  • Multiple sclerosis
  • Total lymphoid irradiation

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