The effects of platelet-derived growth factor and receptor on normal and neoplastic human ovarian surface epithelium

Michael B. Dabrow, Michelle R. Francesco, Francis X. McBrearty, Salvatore Caradonna

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89 Scopus citations

Abstract

Several growth factors have been shown to stimulate or inhibit the growth of human ovarian surface epithelial (HOSE) cells. Platelet-derived growth factor (PDGF) is likely to be released onto the ovarian surface epithelium during follicular wound repair. We undertook the evaluation of this factor and its receptor in normal and malignant ovarian cells. Objectives. The goal of this study was to evaluate the response of HOSE cells to PDGF in vitro and identify PDGF receptors on normal and malignant ovarian epithelial cells. In addition, we wanted to examine the prognostic value of the PDGF receptors in clinical specimens. Methods. Normal HOSE cells were cultured and growth response to PDGF assayed by 3H uptake. PDGF receptor status on HOSE cells, established ovarian carcinoma cell lines, and paraffin tissue was performed by immunohistologic techniques. Data on ovarian cancer patients relapse-free survival (RFS) were abstracted from the Lankenau Hospital Tumor Registry and RFS was plotted using the Kaplan-Meier method. Results. HOSE cells increased 3H uptake in a dose-dependent manner in response to PDGF. HOSE cells stain positively for both α and β receptors, as does the chemotherapy naive cell line A2780. The platinum-resistant CP30 cell line loses PDGF α staining. Of 21 ovarian cancer specimens, only 1 retained PDGF α receptors while 8 retained PDGF β receptors. Those patients positive for PDGF receptor β had a significantly longer relapse-free survival than PDGF β receptor-negative patients. Conclusions. PDGF enhances the growth of HOSE cells in vitro and may play a role in ovarian cancer development. Patients whose tumors retain PDGF receptor β staining positivity have a prolonged relapse-free survival.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
JournalGynecologic Oncology
Volume71
Issue number1
DOIs
StatePublished - Oct 1998

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology
  • Oncology

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