TY - JOUR
T1 - The gene and messenger RNA for adenovirus polypeptide IX
AU - Pettersson, Ulf
AU - Mathews, Michael B.
N1 - Funding Information: We thank R. Greene and R. Roberts for providing the restriction endonucleases, T. Maniatis for polynucleotide kinase and N. Harter for a sample of “early” proteins. The technical assistance of S. Weirich, I. Wendel and G. I. Lindh is gratefully acknowledged. This investigation was supported by a NCI Cancer Center Grant to Cold Spring Harbor Laboratory and by grants from the Swedish Medical Research Council. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 16 U.S.C. Section 1734 solely to indicate this fact.
PY - 1977/11
Y1 - 1977/11
N2 - A small RNA species, distinct from the VA RNAs, has been identified in HeLa cells infected with adenovirus type 2. The RNA, which has been purified using a novel screening procedure, is polyadenylated, sediments at 9S and has an estimated length of 550 nucleotides. In a cell-free translation system, the 9S RNA directs the synthesis of virion polypeptide IX, molecular weight 12,000 daltons. The location of its gene has been established by hybridization of the RNA to fragments of viral DNA produced by cleavage with restriction endonucleases: it spans position 10.0 on the r strand of the viral genome. These results unexpectedly place the gene for a "late" protein within a region of the genome which is transcribed early during infection.
AB - A small RNA species, distinct from the VA RNAs, has been identified in HeLa cells infected with adenovirus type 2. The RNA, which has been purified using a novel screening procedure, is polyadenylated, sediments at 9S and has an estimated length of 550 nucleotides. In a cell-free translation system, the 9S RNA directs the synthesis of virion polypeptide IX, molecular weight 12,000 daltons. The location of its gene has been established by hybridization of the RNA to fragments of viral DNA produced by cleavage with restriction endonucleases: it spans position 10.0 on the r strand of the viral genome. These results unexpectedly place the gene for a "late" protein within a region of the genome which is transcribed early during infection.
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U2 - https://doi.org/10.1016/0092-8674(77)90274-4
DO - https://doi.org/10.1016/0092-8674(77)90274-4
M3 - Article
C2 - 922891
VL - 12
SP - 741
EP - 750
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -