The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells

TY - JOUR

T1 - The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells

AU - Stuparevic, Igor

AU - Becker, Emmanuelle

AU - Law, Michael J.

AU - Primig, Michael

N1 - Publisher Copyright: © 2015 Federation of European Biochemical Societies . Published by Elsevier B.V. All rights reserved.

PY - 2015/4/2

Y1 - 2015/4/2

N2 - The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5′-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5′-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.

AB - The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5′-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5′-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.

UR - https://www.scopus.com/pages/publications/84925492112

UR - https://www.scopus.com/pages/publications/84925492112#tab=citedBy

U2 - 10.1016/j.febslet.2015.02.022

DO - 10.1016/j.febslet.2015.02.022

M3 - Article

C2 - 25728275

SN - 0014-5793

VL - 589

SP - 924

EP - 932

JO - FEBS Letters

JF - FEBS Letters

IS - 8

ER -