The identification of a novel cDNA preferentially expressed in the olfactory-limbic system of the adult rat

Dale G. Schaar, Monica R. Varia, Stella Elkabes, Lakshmi Ramakrishnan, Cheryl F. Dreyfus, Ira B. Black

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

To analyze cell-specific brain gene expression, we have developed a PCR-based subtractive hybridization cloning method utilizing trace starting material, allowing isolation of novel genes expressed under specific conditions. Our previous studies indicated that local substantia nigra (SN) type 1 astrocytes elaborate an array of trophic molecules which support the survival of SN dopaminergic neurons. Therefore, the current study focused on astrocyte gene expression utilizing a type 1 astrocyte-enriched cDNA library. We report initial characterization of a novel cDNA, designated AT1-46, that is preferentially expressed in the olfactory-limbic system of the adult rat brain. Although AT1-46 is expressed widely in the periphery, it is regulated both developmentally and in a cell-specific fashion in the brain. Structurally, AT1-46 is predicted to encode a highly α-helical molecule with several domains of potential coiled coil formation, and exhibits a 28% amino acid sequence identity with the intermediate filament-associated protein, trichohyalin.

Original languageEnglish (US)
Pages (from-to)217-228
Number of pages12
JournalBrain Research
Volume721
Issue number1-2
DOIs
StatePublished - May 20 1996

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Molecular Biology
  • Neuroscience(all)
  • Developmental Biology

Keywords

  • Gene expression
  • Hippocampus
  • Intermediate filament
  • Intermediate filament-associated protein
  • Molecular cloning
  • Olfactory-limbic system
  • Type 1 astrocyte

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